Mapping of opioid receptors using antisense oligodeoxynucleotides: Correlating their molecular biology and pharmacology Journal Article
| Authors: | Pasternak, G. W.; Standifer, K. M. |
| Article Title: | Mapping of opioid receptors using antisense oligodeoxynucleotides: Correlating their molecular biology and pharmacology |
| Abstract: | Understanding the pharmacology of opioid receptors took a major step forward with the cloning of genes that encode four members of the opioid receptor family. Gavril Pasternak and Kelly Standifer show how strategies that use antisense oligodeoxynucleotides can provide a selective approach to correlate the properties of the cloned receptors with their in vivo pharmacological effects, and have confirmed the association of the δ-, μ- and κ1-opioid receptor clones with analgesia mediated through δ-, μ- and κ1-opioid receptors, respectively. Approaches that use antisense oligodeoxynucleotides provide an opportunity to characterize the pharmacology of short cDNA fragments without necessitating the cloning of the entire cDNA and can assess the role of the products of specific exons in a receptor, providing an approach for the study of alternative splicing as demonstrated with the μ- and κ3-opioid receptor clones. © 1995. |
| Keywords: | unclassified drug; exon; review; nonhuman; animal; molecular cloning; cloning, molecular; gene mapping; amino acid sequence; alternative rna splicing; morphine; molecular biology; analgesia; mu opiate receptor; complementary dna; opiate receptor; 3,4 dichloro n methyl n [2 (1 pyrrolidinyl)cyclohexyl]benzeneacetamide; enkephalin[2,5 dextro penicillamine]; oligonucleotides, antisense; peptide mapping; delta opiate receptor; receptor subtype; cyclazocine; opiate antagonist; kappa opiate receptor; naloxonazine; antisense oligodeoxynucleotide; enkephalin[2 dextro alanine 4 methylphenylalanine 5 glycine]; opiate agonist; receptors, opioid; human; priority journal; etorphine; nalorphine; 6 deoxy 6 benzoylhydrazido n allyl 14 hydroxyldihydromorphinone; opiate receptor affecting agent |
| Journal Title: | Trends in Pharmacological Sciences |
| Volume: | 16 |
| Issue: | 10 |
| ISSN: | 0165-6147 |
| Publisher: | Cell Press |
| Date Published: | 1995-10-01 |
| Start Page: | 344 |
| End Page: | 350 |
| Language: | English |
| DOI: | 10.1016/s0165-6147(00)89068-9 |
| PUBMED: | 7491712 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Review -- Export Date: 28 August 2018 -- Source: Scopus |
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