A public genome-scale lentiviral expression library of human ORFs Journal Article
| Authors: | Yang, X.; Boehm, J. S.; Salehi-Ashtiani, K.; Hao, T.; Shen, Y.; Lubonja, R.; Thomas, S. R.; Alkan, O.; Bhimdi, T.; Green, T. M.; Johannessen, C. M.; Silver, S. J.; Nguyen, C.; Murray, R. R.; Hieronymus, H.; Balcha, D.; Fan, C.; Lin, C.; Ghamsari, L.; Vidal, M.; Hahn, W. C.; Hill, D. E.; Root, D. E. |
| Article Title: | A public genome-scale lentiviral expression library of human ORFs |
| Abstract: | Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types. © 2011 Nature America, Inc. All rights reserved. |
| Keywords: | gene expression; gene library; genetic vectors; cloning, molecular; lentivirus; lentivirus vector; genome; open reading frame; open reading frames; genomic library |
| Journal Title: | Nature Methods |
| Volume: | 8 |
| Issue: | 8 |
| ISSN: | 1548-7091 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2011-08-01 |
| Start Page: | 659 |
| End Page: | 661 |
| Language: | English |
| DOI: | 10.1038/nmeth.1638 |
| PROVIDER: | scopus |
| PUBMED: | 21706014 |
| PMCID: | PMC3234135 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 3 October 2011" - "Source: Scopus" |
