The relative distribution of B35 alleles and their IEF isotypes in a HLA‐B35‐positive population Journal Article
| Authors: | Ragupathi, G.; Cereb, N.; Yang, S. Y. |
| Article Title: | The relative distribution of B35 alleles and their IEF isotypes in a HLA‐B35‐positive population |
| Abstract: | Abstract: The HLA‐B35 serotype represents a group of antigens detectable by IEF, cytotoxic T cells, and by sequencing analysis. Four isotypes and eight alleles have been thus far reported. We have determined the relative frequencies of these B35 subtypes in a group of 203 unrelated people. Dot blot hybridization of PCR amplified products was performed using 23 sequence‐specific oligo probes designed based on the EMBL HLA class I sequence database. The amplification was achieved by a pair of group‐specific primers, producing ̃600 bp fragments. By hybridization pattern analysis, we found that four alleles represent over 95% of the B35+ population, with relative frequency of 48.2% for B*3501, 23.7% for B*3502, 15.2% for B*3503, and 8.0% for B*3508. We also identified 3 individuals with B*3504 and one with B*3505, and seven samples with new patterns. B*3501 and B*3503 exactly correlated with the most common isotype B35.3, B*3502 and B*3504 with B35.2, B*3508 may be the B35.1 IEF isotype. The B*3505 was identified from an individual with B35 IEF variant form. Our study shows that the B35 antigen has a wide distribution of alleles, and that many more B35‐related alleles may yet to be uncovered. Copyright © 1995 Munksgaard |
| Keywords: | controlled study; human cell; major clinical study; polymerase chain reaction; allele; gene amplification; alleles; gene frequency; amino acid sequence; molecular sequence data; population; hla-b35 antigen; base sequence; immunophenotyping; hla b antigen; isoantigens; hla system; isoelectric focusing; oligonucleotide probe; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; relative frequency; dot hybridization; hla‐b35; ief isotype; oligo typing |
| Journal Title: | Tissue Antigens |
| Volume: | 46 |
| Issue: | 1 |
| ISSN: | 0001-2815 |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| Date Published: | 1995-07-01 |
| Start Page: | 24 |
| End Page: | 31 |
| Language: | English |
| DOI: | 10.1111/j.1399-0039.1995.tb02472.x |
| PUBMED: | 7482493 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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