Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy Journal Article
| Authors: | Taldone, T.; Gomes DaGama, E. M.; Zong, H.; Sen, S.; Alpaugh, M. L.; Zatorska, D.; Alonso Sabadell, R.; Guzman, M. L.; Chiosis, G. |
| Article Title: | Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy |
| Abstract: | Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5] oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations. © 2011 Elsevier Ltd. All rights reserved. |
| Keywords: | unclassified drug; human cell; flow cytometry; fluorescent dye; drug structure; cell population; drug synthesis; heat shock protein 90 inhibitor; heat shock protein 90; fluorescence microscopy; chemical structure; fluorescein isothiocyanate; purine; pu h 71; pu-h71; fluorescent hsp90 inhibitor; 4 nitrobenzo[1,2,5]oxadiazole; red shifted dye sulforhodamine 101 |
| Journal Title: | Bioorganic & Medicinal Chemistry Letters |
| Volume: | 21 |
| Issue: | 18 |
| ISSN: | 0960-894X |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 2011-09-15 |
| Start Page: | 5347 |
| End Page: | 5352 |
| Language: | English |
| DOI: | 10.1016/j.bmcl.2011.07.026 |
| PROVIDER: | scopus |
| PMCID: | PMC3175602 |
| PUBMED: | 21802945 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 3 October 2011" - "CODEN: BMCLE" - "Source: Scopus" |
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15Gomes-Dagama -
19Alpaugh -
279Chiosis -
93Taldone -
17
Zatorska
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