Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy Journal Article


Authors: Taldone, T.; Gomes DaGama, E. M.; Zong, H.; Sen, S.; Alpaugh, M. L.; Zatorska, D.; Alonso Sabadell, R.; Guzman, M. L.; Chiosis, G.
Article Title: Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy
Abstract: Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5] oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations. © 2011 Elsevier Ltd. All rights reserved.
Keywords: unclassified drug; human cell; flow cytometry; fluorescent dye; drug structure; cell population; drug synthesis; heat shock protein 90 inhibitor; heat shock protein 90; fluorescence microscopy; chemical structure; fluorescein isothiocyanate; purine; pu h 71; pu-h71; fluorescent hsp90 inhibitor; 4 nitrobenzo[1,2,5]oxadiazole; red shifted dye sulforhodamine 101
Journal Title: Bioorganic & Medicinal Chemistry Letters
Volume: 21
Issue: 18
ISSN: 0960-894X
Publisher: Pergamon-Elsevier Science Ltd  
Date Published: 2011-09-15
Start Page: 5347
End Page: 5352
Language: English
DOI: 10.1016/j.bmcl.2011.07.026
PROVIDER: scopus
PMCID: PMC3175602
PUBMED: 21802945
DOI/URL:
Notes: --- - "Export Date: 3 October 2011" - "CODEN: BMCLE" - "Source: Scopus"
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