A conserved downstream element defines a new class of RNA polymerase II promoters Journal Article

  


Authors: Ince, T. A.; Scotto, K. W.
Article Title: A conserved downstream element defines a new class of RNA polymerase II promoters
Abstract: Although many TATA-less promoters transcribed by RNA polymerase II initiate transcription at multiple sites, the regulation of multiple start site utilization is not understood. Beginning with the prediction that multiple start site promoters may share regulatory features and using the P- glycoprotein promoter (which can utilize either a single or multiple transcription start site(s)) as a model, several promoters with analogous transcription windows were grouped and searched for the presence of a common DNA element. A downstream protein-binding sequence, MED-1 (Multiple start site Element Downstream), was found in the majority of promoters analyzed. Mutation of this element within the P-glycoprotein promoter reduced transcription by selectively decreasing utilization of downstream start sites. We propose that a new class of RNA polymerase II promoters, those that can utilize a distinctive window of multiple start sites, is defined by the presence of a downstream MED-1 element.
Keywords: gene mutation; promoter region; nonhuman; protein analysis; animal cell; animals; cell line; transcription, genetic; transfection; animalia; conserved sequence; molecular sequence data; recombinant proteins; base sequence; mutagenesis, site-directed; binding sites; thymidine kinase; sequence homology, nucleic acid; thymidylate synthase; luciferases; rna polymerase ii; dna determination; p-glycoprotein; rna transcription; oligodeoxyribonucleotides; regulatory sequences, nucleic acid; tata box; hypoxanthine phosphoribosyltransferase; promoter regions (genetics); humans; priority journal; article; hydroxymethylglutaryl coa reductases
Journal Title: Journal of Biological Chemistry
Volume: 270
Issue: 51
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 1995-12-22
Start Page: 30249
End Page: 30252
Language: English
DOI: 10.1074/jbc.270.51.30249
PUBMED: 8530439
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029586499&doi=10.1074%2fjbc.270.51.30249&partnerID=40&md5=a023993cc4856d703ccee03fd44d3cfc
Notes: Article -- Export Date: 28 August 2018 -- Source: Scopus
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MSK Authors
  1. Kathleen Weihs Scotto
    56 Scotto
  2. Tan A Ince
    11 Ince
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