N-glycosylation of the human granulocyte-macrophage colony-stimulating factor receptor α subunit is essential for ligand binding and signal transduction Journal Article
| Authors: | Ding, D. X. H.; Vera, J. C.; Heaney, M. L.; Golde, D. W. |
| Article Title: | N-glycosylation of the human granulocyte-macrophage colony-stimulating factor receptor α subunit is essential for ligand binding and signal transduction |
| Abstract: | The α subunit of the receptor for human granulocyte-macrophage colony- stimulating factor (GM-CSF) is a glycoprotein containing 11 potential N- glycosylation sites in the extracellular domain. We examined the role of N- glycosylation on α subunit membrane localization and function. Tunicamycin, an N-glycosylation inhibitor, markedly inhibited GM-CSF binding, GM-CSF- induced deoxyglucose uptake, and protein tyrosine phosphorylation in HL- 60(eos) cells but did not affect cell surface expression of the α subunit as detected by an anti-α subunit monoclonal antibody. In COS cells expressing the α subunit and treated with tunicamycin, N-unglycosylated α subunit was expressed and transported to the cell surface but was not capable of binding GM-CSF. High affinity binding in COS cells expressing both α and β subunits was also blocked by tunicamycin treatment. These studies indicate that N- linked oligosaccharides are essential for α subunit ligand binding and signaling by the human GM-CSF receptor. |
| Keywords: | signal transduction; protein phosphorylation; human cell; binding affinity; protein localization; animal; granulocyte macrophage colony stimulating factor; cell line; transfection; cercopithecus aethiops; endoplasmic reticulum; kinetics; recombinant proteins; cell membrane; glycosylation; immunoblotting; ligands; binding site; protein folding; antigen binding; ligand binding; granulocyte-macrophage colony-stimulating factor; hl-60 cells; receptors, granulocyte-macrophage colony-stimulating factor; tunicamycin; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; macromolecular systems |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 270 |
| Issue: | 41 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1995-10-13 |
| Start Page: | 24580 |
| End Page: | 24584 |
| Language: | English |
| DOI: | 10.1074/jbc.270.41.24580 |
| PUBMED: | 7592677 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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