Resolution of the facilitated transport of dehydroascorbic acid from its intracellular accumulation as ascorbic acid Journal Article

  

Authors:	Vera, J. C.; Rivas, C. I.; Velásquez, F. V.; Zhang, R. H.; Concha, I. I.; Golde, D. W.
Article Title:	Resolution of the facilitated transport of dehydroascorbic acid from its intracellular accumulation as ascorbic acid
Abstract:	We performed a detailed kinetic analysis of the uptake of dehydroascorbic acid by HL-60 cells under experimental conditions that enabled the differentiation of dehydroascorbic acid transport from the intracellular reduction/accumulation of ascorbic acid. Immunoblotting and immunolocalization experiments identified GLUT1 as the main glucose transporter expressed in the HL-60 cells. Kinetic analysis allowed the identification of a single functional activity involved in the transport of dehydroascorbic acid in the HL-60 cells. Transport was inhibited in a competitive manner by both 3-0-methyl-D-glucose and 2-deoxy-D-glucose. In turn, dehydroascorbic acid competitively inhibited the transport of both sugars. A second functional component identified in experiments measuring the accumulation of ascorbic acid appears to be associated with the intracellular reduction of dehydroascorbic acid to ascorbic acid and is not directly involved in the transport of dehydroascorbic acid via GLUT1. Transport of dehydroascorbic acid by HL-60 cells was independent of the presence of external Na +, whereas the intracellular accumulation of ascorbic acid was found to be a Na +-sensitive process. Thus, the transport of dehydroascorbic acid via glucose transporters is a Na +-independent process which is kinetically and biologically separable from the reduction of dehydroascorbic acid to ascorbic acid and its subsequent intracellular accumulation.
Keywords:	human cell; models, biological; cell line; intracellular transport; kinetics; ascorbic acid; sodium; dehydroascorbic acid; oxidation-reduction; reduction; competitive inhibition; binding, competitive; glucose transporter; deoxyglucose; biological transport, active; cell strain hl 60; sodium ion; monosaccharide transport proteins; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; intracellular fluid; 3 methylglucose; 3-o-methylglucose; methylglucosides
Journal Title:	Journal of Biological Chemistry
Volume:	270
Issue:	40
ISSN:	0021-9258
Publisher:	American Society for Biochemistry and Molecular Biology
Date Published:	1995-10-06
Start Page:	23706
End Page:	23712
Language:	English
DOI:	10.1074/jbc.270.40.23706
PUBMED:	7559541
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028853597&doi=10.1074%2fjbc.270.40.23706&partnerID=40&md5=4c6ed92ef3d9d1ff9606595eed1475c0
Notes:	Article -- Export Date: 28 August 2018 -- Source: Scopus

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