Transfection of wild-type but not mutant p53 induces early monocytic differentiation in HL60 cells and increases their sensitivity to stress Journal Article
| Authors: | Banerjee, D.; Lenz, H. J.; Schnieders, B.; Manno, D. J.; Ju, J. F.; Spears, C. P.; Hochhauser, D.; Danenberg, K.; Danenberg, P.; Bertino, J. R. |
| Article Title: | Transfection of wild-type but not mutant p53 induces early monocytic differentiation in HL60 cells and increases their sensitivity to stress |
| Abstract: | HL60 cells, which lack the p53 gene due to a deletion, were used as an in vitro model system to study the effect of wild-type p53 gene expression on hematopoietic differentiation. We transfected HL60 cells with wild-type p53 and two mutant p53 cDNAs encoding the Val to Ala mutation at codon 143 and the Arg to Trp mutation at codon 248. Flow cytometry, growth, and cytochemical analysis for α-napthyl-butyrate esterase activity and nitroblue tetrazolium reduction indicated that wild-type p53 but not mutant p53 induced early monocytic differentiation in the transfected HL60 cells without terminal growth arrest. The wild-type p53 transfectants did not differentiate along the granulocytic pathway, even when induced with 1.25% DMSO for 6 days; rather, these cells resembled monocytic cells, confirming that wild-type p53 transfection caused these cells to become committed to differentiate along the monocytic pathway. HL60 cells transfected with wild-type p53 were more sensitive to stress, such as growth in serum-depleted medium and exposure to a chemotherapeutic agent, etoposide. |
| Keywords: | nonhuman; flow cytometry; sensitivity and specificity; blood proteins; animal cell; cell division; gene expression; amino acid substitution; etoposide; cell differentiation; enzyme activity; transfection; protein p53; tumor suppressor gene; dimethyl sulfoxide; molecular sequence data; genetic transfection; base sequence; monocytes; cytochemistry; culture media; antigens, surface; esterase; tetradecanoylphorbol acetate; hl-60 cells; cell strain hl 60; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; nitroblue tetrazolium |
| Journal Title: | Cell Growth & Differentiation |
| Volume: | 6 |
| Issue: | 11 |
| ISSN: | 1044-9523 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1995-11-01 |
| Start Page: | 1405 |
| End Page: | 1413 |
| Language: | English |
| PUBMED: | 8562479 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
