Alternative Pre-mRNA splicing of G protein-coupled receptors Journal Article
| Authors: | Pan, Y. X.; Grinnell, S.; Pasternak, G. W. |
| Article Title: | Alternative Pre-mRNA splicing of G protein-coupled receptors |
| Abstract: | Alternative pre-mRNA splicing involves editing of a gene to generate a number of different mRNAs and proteins. It provides a mechanism for only 20,000 genes to generate hundreds of thousands of proteins. Like other proteins, it is estimated that 50% of G protein-coupled receptors undergo alternative splicing. While most commonly involving either the N-terminus or C-terminus, some variants have modifications in the interior of the receptor. Alternative splicing generates functionally distinct variants, due to an intrinsic difference in transduction or location. These features are well illustrated by the mu opioid receptor gene, OPRM1, which undergoes extensive alternative splicing. © 2011 Springer Science+Business Media, LLC. |
| Keywords: | nonhuman; protein localization; gene targeting; reverse transcription polymerase chain reaction; protein processing; alternative splicing; splice variants; alternative rna splicing; protein structure; structure analysis; protein variant; g protein coupled receptor; mu opiate receptor; receptor gene; receptor binding; knockout mouse; gene structure; pharmacological blocking; mor-1; mu opioid receptor; oprm1; truncated variants; antisense oligodeoxynucleotide; oprm1 gene |
| Journal Title: | Neuromethods |
| Volume: | 60 |
| ISSN: | 0893-2336 |
| Publisher: | Springer Science + Business Media, LLC |
| Date Published: | 2011-01-01 |
| Start Page: | 19 |
| End Page: | 34 |
| Language: | English |
| DOI: | 10.1007/978-1-61779-179-6_2 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Chapter 2 in "Methods for the Discovery and Characterization of G Protein-Coupled Receptors" (ISBN: 978-1-61779-178-9) -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work