Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase Journal Article
| Authors: | Fu, Y. V.; Yardimci, H.; Long, D. T.; Ho, V.; Guainazzi, A.; Bermudez, V. P.; Hurwitz, J.; Van Oijen, A.; Schärer, O. D.; Walter, J. C. |
| Article Title: | Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase |
| Abstract: | The eukaryotic replicative DNA helicase, CMG, unwinds DNA by an unknown mechanism. In some models, CMG encircles and translocates along one strand of DNA while excluding the other strand. In others, CMG encircles and translocates along duplex DNA. To distinguish between these models, replisomes were confronted with strand-specific DNA roadblocks in Xenopus egg extracts. An ssDNA translocase should stall at an obstruction on the translocation strand but not the excluded strand, whereas a dsDNA translocase should stall at obstructions on either strand. We found that replisomes bypass large roadblocks on the lagging strand template much more readily than on the leading strand template. Our results indicate that CMG is a 3′ to 5′ ssDNA translocase, consistent with unwinding via "steric exclusion." Given that MCM2-7 encircles dsDNA in G1, the data imply that formation of CMG in S phase involves remodeling of MCM2-7 from a dsDNA to a ssDNA binding mode. © 2011 Elsevier Inc. |
| Keywords: | unclassified drug; nonhuman; dna replication; dna synthesis; cell cycle protein; animals; cell cycle s phase; models, biological; protein; dna; double stranded dna; eukaryota; 5' untranslated region; chromatin immunoprecipitation; 3' untranslated region; helicase; single stranded dna; dna, single-stranded; dna binding; s phase; dna helicases; gins protein; eukaryote; xenopus; minichromosome maintenance protein 2; minichromosome maintenance protein 3; minichromosome maintenance protein 4; minichromosome maintenance protein 5; minichromosome maintenance protein 6; minichromosome maintenance protein 7; cell cycle protein 45 |
| Journal Title: | Cell |
| Volume: | 146 |
| Issue: | 6 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2011-09-16 |
| Start Page: | 931 |
| End Page: | 941 |
| Language: | English |
| DOI: | 10.1016/j.cell.2011.07.045 |
| PROVIDER: | scopus |
| PUBMED: | 21925316 |
| PMCID: | PMC3209622 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 3 October 2011" - "CODEN: CELLB" - "Source: Scopus" |
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