Inducible disruption of the c-myb gene allows allogeneic bone marrow transplantation without irradiation Journal Article
| Authors: | Stremmel, C.; Schuchert, R.; Schneider, V.; Weinberger, T.; Thaler, R.; Messerer, D.; Helmer, S.; Geissmann, F.; Frampton, J.; Massberg, S.; Schulz, C. |
| Article Title: | Inducible disruption of the c-myb gene allows allogeneic bone marrow transplantation without irradiation |
| Abstract: | Allogeneic bone marrow (BM) transplantation enables the in vivo functional assessment of hematopoietic cells. As pre-conditioning, ionizing radiation is commonly applied to induce BM depletion, however, it exerts adverse effects on the animal and can limit experimental outcome. Here, we provide an alternative method that harnesses conditional gene deletion to ablate c-myb and thereby deplete BM cells, hence allowing BM substitution without other pre-conditioning. The protocol results in a high level of blood chimerism after allogeneic BM transplantation, whereas immune cells in peripheral tissues such as resident macrophages are not replaced. Further, mice featuring a low chimerism after initial transplantation can undergo a second induction cycle for efficient deletion of residual BM cells without the necessity to re-apply donor cells. In summary, we present an effective c-myb-dependent genetic technique to generate BM chimeras in the absence of irradiation or other methods for pre-conditioning. © 2018 Elsevier B.V. |
| Keywords: | nonhuman; mouse; bone marrow; animal experiment; allogenic bone marrow transplantation; transplantation; chimera; gene disruption; cd11b antigen; hematopoietic stem cells; polyinosinic polycytidylic acid; hematopoiesis; bone marrow cell; monocyte; macrophage; lymphocyte; oncogene c myb; immunocompetent cell; granulocyte; c-myb; bone marrow depression; priority journal; article; radiation chimera; conditional deletion; receptor type tyrosine protein phosphatase c |
| Journal Title: | Journal of Immunological Methods |
| Volume: | 457 |
| ISSN: | 0022-1759 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2018-06-01 |
| Start Page: | 66 |
| End Page: | 72 |
| Language: | English |
| DOI: | 10.1016/j.jim.2018.03.016 |
| PROVIDER: | scopus |
| PUBMED: | 29630967 |
| PMCID: | PMC6022737 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2018 -- Source: Scopus |
