Expression and mutational analysis of P53 in stage IB and IIA cervical cancers Journal Article
| Authors: | Benjamin, I.; Saigo, P.; Finstad, C.; Takahashi, H.; Federici, M.; Rubin, S. C.; Boyd, J. |
| Article Title: | Expression and mutational analysis of P53 in stage IB and IIA cervical cancers |
| Abstract: | OBJECTIVE: This study evaluates overexpression of the p53 protein and point mutation in the P53 gene in a group of patients with stage IB and IIA cervical cancer. STUDY DESIGN: We reviewed the medical records of all patients who underwent radical hysterectomy for the treatment of stage IB and IIA cervical cancer between 1980 and 1985 at Memorial Sloan-Kettering Cancer Center. Overexpression of p53 protein was determined with the use of immunohistochemistry on fixed and paraffin-embedded tissue. Two blocks were selected for each tumor, and tissue sections from each block were tested with both monoclonal (Ab-6) and polyclonal (CM-1) anti-p53 antibodies. Molecular analysis for determination of specific P53 gene mutations was performed with single-strand conformation polymorphism analysis. A group of 132 patients was identified for inclusion in the study. RESULTS: Fifty-eight of 132 tumors (44%) showed overexpression of the p53 protein and were subjected to molecular analysis. Discrepancy between pairs of blocks (7/132, 5.3%) and between antibodies for the same block (5/264, 1.9%) was uncommon. High-level overexpression was rare (5/132, 3.8%). No difference in survival was found on the basis of overexpression of p53 protein, Only one of the 58 cases (1/58, 1.7%) that showed overexpression of the p53 protein exhibited a point mutation (exon 8) in P53 by single-strand conformation polymorphism. This case had a low level of overexpression of p53 protein on immunohistochemistry. CONCLUSIONS: Low levels of overexpression of p53 were frequently seen in early cervical cancers (40/132, 30%). However, mutation of the P53 gene was rarely seen in these tumors. Overexpression of p53 protein as detected by immunohistochemistry is not predictive of a somatic mutation in the P53 gene in cervical cancer. Molecular analysis is required for confirmation of P53 mutations in these tumors. |
| Keywords: | immunohistochemistry; gene; protein; p53; mutant; cervical cancer; polymorphism; overexpression; state; carcinoma cell-lines; human papillomavirus infection; single-strand conformation |
| Journal Title: | American Journal of Obstetrics and Gynecology |
| Volume: | 175 |
| Issue: | 5 |
| ISSN: | 0002-9378 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1996-11-01 |
| Start Page: | 1266 |
| End Page: | 1271 |
| Language: | English |
| ACCESSION: | WOS:A1996VU51400023 |
| DOI: | 10.1016/s0002-9378(96)70039-x |
| PROVIDER: | wos |
| PUBMED: | 8942499 |
| Notes: | Article -- Source: Wos |
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