Differential effects of ceramide on cell viability and extracellular matrix remodeling in keratinocytes and fibroblasts Journal Article

Authors: Philips, N.; Tuason, M.; Chang, T.; Lin, Y.; Tahir, M.; Rodriguez, S. G.
Article Title: Differential effects of ceramide on cell viability and extracellular matrix remodeling in keratinocytes and fibroblasts
Abstract: Ceramides are used in skin care and treatment of dermatological diseases. Cell viability and extracellular matrix (ECM) remodeling are important parameters in skin health. Skin photoaging, from exposure to ultraviolet radiation, is associated with epidermal hyperplasia and dermal ECM atrophy caused by alterations in expression of matrixmetalloproteinases (MMPs), elastin and transforming growth factor-β (TGF-β). The purpose of this study was to determine the effects of c2-ceramide (ceramide) on the cell viability and expression of TGF-β, MMP-1 and elastin in cultured keratinocytes and fibroblasts. Ceramide inhibited keratinocyte cell viability by apoptosis and stimulated expression of elastin, MMP-1 and TGF-β, suggesting improved epidermal functioning by a TGF-β mechanism. Conversely, ceramide stimulated fibroblast cell growth at the lower concentrations and inhibited the expression of MMP-1, elastin and TGF-β, which indicates a predominantly beneficial effect in the prevention of photoaging-associated dermal alterationby TGF-β and non-TGF-β mechanisms. The regulation of MMP-1 expression by ceramide was transcriptionally mediated and via the activator protein-1 sequence in both keratinocytes and fibroblasts. The study delineates the specific, though differential, beneficial effects of ceramide in the prevention of epidermal hyperplasia and dermal ECM remodeling, associated with photoaging. Copyright © 2009 S. Karger AG, Basel.
Keywords: controlled study; protein expression; human cell; ultraviolet radiation; cell viability; cell survival; cells, cultured; apoptosis; cell growth; transforming growth factor beta; drug effect; keratinocyte; radiation exposure; extracellular matrix; cell culture; promoter regions, genetic; newborn; matrix metalloproteinase; fibroblast; fibroblasts; keratinocytes; interstitial collagenase; transforming growth factor alpha; matrix metalloproteinase 1; photoaging; c2-ceramide; elastin; matrixmetalloproteinases-1; transforming growth factor-β; ceramide; epidermis hyperplasia; skin atrophy; ceramides
Journal Title: Skin Pharmacology and Physiology
Volume: 22
Issue: 3
ISSN: 1660-5527
Publisher: S. Karger AG  
Date Published: 2009-04-01
Start Page: 151
End Page: 157
Language: English
DOI: 10.1159/000208168
PUBMED: 19276645
PROVIDER: scopus
Notes: --- - "Export Date: 30 November 2010" - "CODEN: SPPKC" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Salvador Gonzalez Rodriguez
    203 Rodriguez