STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo Journal Article
| Authors: | Bazzi, H.; Soroka, E.; Alcorn, H. L.; Anderson, K. V.; Hogan, B. L. M. |
| Article Title: | STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo |
| Abstract: | Regulated mesoderm migration is necessary for the proper morphogenesis and organ formation during embryonic development. Cell migration and its dependence onthe cytoskeleton and signaling machines have been studied extensively in cultured cells; in contrast, remarkably little is known about the mechanisms that regulate mesoderm cell migration in vivo. Here, we report the identification and characterization of a mouse mutation in striatin-interacting protein 1 (Strip1) that disrupts migration of the mesoderm after the gastrulation epithelial-to-mesenchymal transition (EMT). STRIP1 is a core component of the biochemically defined mammalian striatin-interacting phosphatases and kinase (STRIPAK) complexes that appear to act through regulation of protein phosphatase 2A (PP2A), but their functions in mammals in vivo have not been examined. Strip1-null mutants arrest development at midgestation with profound disruptions in the organization of the mesoderm and its derivatives, including a complete failure of the anterior extension of axial mesoderm. Analysis of cultured mesoderm explants and mouse embryonic fibroblasts from null mutants shows that the mesoderm migration defect is correlated with decreased cell spreading, abnormal focal adhesions, changes in the organization of the actin cytoskeleton, and decreased velocity of cell migration. The results show that STRIPAK complexes are essential for cell migration and tissue morphogenesis in vivo. |
| Keywords: | immunohistochemistry; controlled study; unclassified drug; nonhuman; animal cell; mouse; phenotype; mesenchyme cell; basement membrane; phosphatase; embryo; sonic hedgehog protein; protein; morphogenesis; uvomorulin; gene mapping; nerve cell adhesion molecule; western blotting; cell migration; fibroblast; f actin; mesoderm; phosphotransferase; transcription factor sox2; mouse embryo; gastrulation; transcription factor sox17; hepatocyte nuclear factor 3beta; primitive streak; epithelial mesenchymal transition; phosphoprotein phosphatase 2a; actin filament; focal adhesion; pp2a; next generation sequencing; cell spreading; sanger sequencing; priority journal; article; strip1; stripak; aminomethyltransferase; laminin1 protein; snail1 protein; striatin interacting phosphatase and kinase; striatin interacting protein 1; mesodermal cell |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 114 |
| Issue: | 51 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2017-12-19 |
| Start Page: | E10928 |
| End Page: | E10936 |
| Language: | English |
| DOI: | 10.1073/pnas.1713535114 |
| PROVIDER: | scopus |
| PMCID: | PMC5754794 |
| PUBMED: | 29203676 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 February 2018 -- Source: Scopus |
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