Effect of interleukin 12 on tumor induction by 3-methylcholanthrene Journal Article
| Authors: | Noguchi, Y.; Jungbluth, A.; Richards, E. C.; Old, L. J. |
| Article Title: | Effect of interleukin 12 on tumor induction by 3-methylcholanthrene |
| Abstract: | Interleukin (IL)-12 has strong antitumor activity in transplantable tumor systems in the mouse. The present study was designed to determine whether tumor induction by 3-methylcholanthrene (3-MC), a carcinogenic hydrocarbon, can be inhibited by IL-12, BALB/cBy mice were injected subcutaneously with 25 μg or 100 μg of 3-MC and treated with 100 ng, 10 ng, or 1 ng of IL-12 for 5 days a week for 18 weeks, with a schedule of 3 weeks on and 1 week off. In mice injected with 25 μg of 3-MC, treatment with 100 ng of IL-12 delayed tumor appearance and reduced tumor incidence. Tumor appearance was also delayed in mice injected with 100 μg of 3-MC and treated with 100 ng of IL-12, but the final tumor incidence was the same as in non- IL-12-treated mice. In contrast to the characteristically round, hard, well- circumscribed, and protruding tumor induced by 3-MC, a percentage of tumors induced in IL-12-treated mice had atypical characteristics: flat, soft, and invasive. Atypical tumors had a longer latent period and were more frequently seen in mice injected with 100 μg of 3-MC and treated with 100 ng of IL-12. Interferon γ, IL-10, and tumor necrosis factor could be induced throughout the treatment period by IL-12, indicating that repeated injections of IL-12 do not induce a state of tachyphylaxis. High production of interferon γ by CD8 T cells and a TH2 → TH1 or TH0 shift in the cytokine secretion profile of CDd T cells were also seen in the IL-12-treated mice. IL-12 provides a powerful new way to explore the defensive role of the immune system in tumorigenesis. |
| Keywords: | nonhuman; cd8-positive t-lymphocytes; animal cell; mouse; animals; mice; cells, cultured; cancer immunotherapy; skin neoplasms; animal experiment; mice, scid; mice, inbred balb c; time factors; animalia; cytokines; tumor necrosis factor-alpha; gamma interferon; anticarcinogenic agents; cd4-positive t-lymphocytes; recombinant proteins; interleukin 12; carcinogens; cancer immunology; nitrites; interleukin-12; interferon type ii; methylcholanthrene; chemical carcinogenesis; cancer; female; priority journal; article; interferon γ; chemically induced tumors; nonspecific immunotherapy |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 93 |
| Issue: | 21 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1996-10-15 |
| Start Page: | 11798 |
| End Page: | 11801 |
| Language: | English |
| DOI: | 10.1073/pnas.93.21.11798 |
| PUBMED: | 8876217 |
| PROVIDER: | scopus |
| PMCID: | PMC38138 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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