Interleukin-1 production following T-cell-depleted and unmodified marrow grafts Journal Article

Authors: Sahdev, I.; O'Reilly, R.; Black, P.; Heller, G.; Hoffmann, M.
Article Title: Interleukin-1 production following T-cell-depleted and unmodified marrow grafts
Abstract: Interleukin-1 (IL-1) production by endotoxin-stimulated cultured monocytes from 31 participants in grafts of marrow depleted of mature cellular elements by treatment with soybean agglutinin and sheep red blood cells (SBA-E+) and 12 recipients of unfractionated bone marrow were studied and compared with normal controls. Patients were studied prior to marrow transplant (BMT) and at 1 month, 2 to 4 months, and 5 to 6 months post-transplant. Deficiencies in IL-1 production (<50 units) were detected in both transplant groups prior to and at 1 month post-MBT. From 2 to 4 months post-transplant, 67% of the recipients of unmodified marrow and 45% of the recipients of SBA-E- marrow grafts produced a normal level of IL-1. By 5 to 6 months post-transplant and thereafter, the proportions of patients exhibiting deficiencies in IL-1 production in each group were equally low. We also evaluated the impact of early deficiencies of IL-1 on engraftment, hematopoietic function, and immunological reconstitution. Deficiencies in IL-1 production persisting to 2 to 4 months post- BMT did not significantly affect the degree of chimerism or the time to recovery of neurotrophil counts to 500/μl in recipients of either unmodified or T-cell-depleted marrow. Platelet recovery during the first 50 days post-transplant was significantly slower in the IL-1-deficient group, but thereafter rebounded, so that by 4 months post-BMT patients with initial deficiencies in IL-1 production archieved levels comparable with those attained by patients with normal production of IL-1. When we looked at the lymphocyte response to phytohemagglutinin (PHA), there was no difference detected among patients with or without IL-1 deficiency receiving unmodified transplants. In contrast, recipients of T- cell-depleted grafts exhibiting a prolonged deficiency of IL-1 experienced a slower rate of recovery of PHA responses. Our results suggest that IL-1 may play an important role in the early expansion of megakaryocytic precursors following an allogeneic marrow transplant and may facilitate the functional development of allogeneic lymphoid progenitors following a T-cell-depleted marrow graft.
Keywords: adolescent; adult; child; clinical article; controlled study; child, preschool; acute granulocytic leukemia; human cell; t lymphocyte; allogenic bone marrow transplantation; chronic myeloid leukemia; acute lymphoblastic leukemia; myelodysplastic syndrome; nonhodgkin lymphoma; infant; graft versus host reaction; hematopoiesis; monocytes; bone marrow transplantation; lymphocyte depletion; interleukin 1; interleukin-1; bmt; t-cell depleted; endotoxins; humans; human; male; female; article; il-1; marrow graft; unmodified marrow graft
Journal Title: Pediatric Hematology and Oncology
Volume: 13
Issue: 1
ISSN: 0888-0018
Publisher: Taylor & Francis Group  
Date Published: 1996-01-01
Start Page: 55
End Page: 67
Language: English
PUBMED: 8718503
PROVIDER: scopus
DOI: 10.3109/08880019609033372
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
Citation Impact
MSK Authors
  1. Glenn Heller
    353 Heller
  2. Richard O'Reilly
    585 O'Reilly
  3. Patricia   Black
    26 Black