MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties Journal Article
| Authors: | Miyazaki, Y.; Sun, X.; Uchida, H.; Zhang, J.; Nimer, S. |
| Article Title: | MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties |
| Abstract: | To isolate novel ETS genes expressed in hematopoietic cells we used a nested, degenerate oligonucleotide PCR based technique to obtain a cDNA fragment that encodes an ETS domain from a human megakaryocytic cell line. This fragment was used to isolate a full length, 4.2 kb cDNA that encodes a 663 amino acid protein which we call MEF (myeloid elf-1-like factor). MEF contains a central ETS domain and is expressed in a variety of myeloid leukemia cell lines and in normal adult hematopoietic tissue such as spleen, thymus and peripheral blood, as well as non-hematopoietic tissue such as ovary, placenta and colon. Using an oligonucleotide binding selection assay, and bacterially expressed MEF, we demonstrated that MEF recognizes a consensus DNA binding sequence nearly identical to elf-1 (..WGGA..) and then showed that MEF specifically bound to known elf-1 binding sites in the human GMCSF and IL-3 promoters. To characterize the functional effect of MEF on transcription, we performed co-transfection experiments in myeloid cells, and T cells, and demonstrated transactivation of both promoters by MEF, but not by elf-1. MEF is a new member of the elf-1/E74 family of ETS proteins, that has DNA binding properties similar to elf-1 but distinct transcriptional activating effects in several hematopoietic cell lineages. |
| Keywords: | adult; human cell; dna-binding proteins; polymerase chain reaction; cell line; protein binding; transcription factor; transcription factors; amino acid sequence; molecular sequence data; sequence homology, amino acid; hematopoietic cell; trans-activators; dna binding; leukemia cell line; dna, complementary; consensus sequence; granulocyte-macrophage colony-stimulating factor; promoter regions (genetics); interleukin-3; humans; human; priority journal; article; transcriptional activator; ets protein; hematopoietic tissue; cytokine genes |
| Journal Title: | Oncogene |
| Volume: | 13 |
| Issue: | 8 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1996-10-17 |
| Start Page: | 1721 |
| End Page: | 1729 |
| Language: | English |
| PUBMED: | 8895518 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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