A nucleolar RNA helicase recognized by autoimmune antibodies from a patient with watermelon stomach disease Journal Article
| Authors: | Valdez, B. C.; Henning, D.; Busch, R. K.; Woods, K.; Flores-Rozas, H.; Hurwitz, J.; Perlaky, L.; Busch, H. |
| Article Title: | A nucleolar RNA helicase recognized by autoimmune antibodies from a patient with watermelon stomach disease |
| Abstract: | Watermelon stomach is characterized by prominent stripes of ectatic vascular tissue in the stomach similar to stripes on a watermelon; in patients with this disorder chronic gastrointestinal bleeding occurs and approximately half of these patients have associated autoimmune disorders. In the serum of one patient, an antinucleolar antibody titer of 1:25 600 was found; the antibodies specifically recognized a ~ 100 kDa nucleolar protein, which we referred to as the 'Gu' protein. Its cDNA was cloned and sequenced. The Gu protein is a member of a new subgroup of RNA helicases, the DEXD box family. Gu protein fused with glutathione S-transferase contains ATP-dependent RNA helicase activity which preferably translocates in the 5' → 3' direction. Its RNA folding activity, RNA-dependent ATPase and dATPase activities, and its translocation direction are similar to those of RNA helicase II. Sequencing of 209 amino acids of RNA helicase II peptides showed 96.7% identity with the cDNA-derived amino acid sequence of the Gu protein. The precise biological roles of this RNA helicase in the biogenesis of ribosomal RNA and the pathogenesis of watermelon disease and autoimmune disorder require further study. |
| Keywords: | human cell; case report; gastrointestinal hemorrhage; nuclear protein; molecular cloning; cloning, molecular; amino acid sequence; molecular sequence data; hybrid protein; glutathione transferase; autoantigens; autoimmunity; antinuclear antibody; rna structure; sequence homology; ribosome rna; autoantibody; autoimmune diseases; adenosine triphosphatase; rna helicase; rna helicases; adenosine triphosphatases; autoantibodies; complementary dna; antibody titer; dna, complementary; nucleolus; cell nucleolus; stomach disease; rna nucleotidyltransferases; humans; human; priority journal; article; vascular tissue; stomach diseases; citrullus lanatus var. lanatus |
| Journal Title: | Nucleic Acids Research |
| Volume: | 24 |
| Issue: | 7 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 1996-04-01 |
| Start Page: | 1220 |
| End Page: | 1224 |
| Language: | English |
| DOI: | 10.1093/nar/24.7.1220 |
| PUBMED: | 8614622 |
| PROVIDER: | scopus |
| PMCID: | PMC145780 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
