An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase α and replicate SV40 DNA in vitro Journal Article
| Authors: | Weisshart, K.; Bradley, M. K.; Weiner, B. M.; Schneider, C.; Moarefi, I.; Fanning, E.; Arthur, A. K. |
| Article Title: | An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase α and replicate SV40 DNA in vitro |
| Abstract: | A peptide encompassing the N-terminal 82 amino acids of simian virus 40 (SV40) large T antigen was previously shown to bind to the large subunit of DNA polymerase α-primase (I. Dornreiter, A. Hoss, A. K. Arthur, and E. Fanning, EMBO J. 9:3329-3336, 1990). We report here that a mutant T antigen, T83-708, lacking residues 2 to 82 retained the ability to bind to DNA polymerase α-primase, implying that it carries a second binding site for DNA polymerase α-primase. The mutant protein also retained ATPase, helicase, and SV40 origin DNA-binding activity. However, its SV40 DNA replication activity in vitro was reduced compared with that of wild-type protein. The reduction in replication activity was accompanied by a lower DNA-binding affinity to SV40 origin sequences and aberrant oligomerization on viral origin DNA. Thus, the first 82 residues of SV40 T antigen are not strictly required for its interaction with DNA polymerase α-primase or for DNA replication function but may play a role in correct hexamer assembly and efficient DNA binding at the origin. |
| Keywords: | human cell; mutation; dna polymerase; binding affinity; dna replication; protein assembly; protein protein interaction; in vitro study; amino terminal sequence; virus large t antigen; simian virus 40; binding site; dna, viral; virus replication; dna binding; antigens, polyomavirus transforming; enzyme subunit; adenosine triphosphatases; oligomerization; dna helicases; deletion mutant; dna polymerase ii; human; priority journal; article |
| Journal Title: | Journal of Virology |
| Volume: | 70 |
| Issue: | 6 |
| ISSN: | 0022-538X |
| Publisher: | American Society for Microbiology |
| Date Published: | 1996-06-01 |
| Start Page: | 3509 |
| End Page: | 3516 |
| Language: | English |
| PUBMED: | 8648684 |
| PROVIDER: | scopus |
| PMCID: | PMC190225 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |