Evaluation of carbon-14-colchicine biodistribution with whole-body quantitative autoradiography in colchicine-sensitive and -resistant xenografts Journal Article
| Authors: | Mehta, B. M.; Levchenko, A.; Rosa, E.; Kim, S. W.; Winnick, S.; Zhang, J. J.; Kalaigian, H.; Larson, S. M. |
| Article Title: | Evaluation of carbon-14-colchicine biodistribution with whole-body quantitative autoradiography in colchicine-sensitive and -resistant xenografts |
| Abstract: | Quantitative autoradiography (QAR) with radiolabeled monoclonal antibodies in xenografted animals has been extensively described in the past, either on individual tissues or on the whole body. We applied whole-body QAR to identify multidrug resistant tumors using 14C-colchicine (14C-CHC). Methods: Two groups of five animals each were xenografted with CHC-sensitive and CHC-resistant human neuroblastoma cells. Animals were injected intravenously with 4 μCi/0.11 μmole 14C-CHC per gram of body weight and sacrificed after 60 min. Whole-body QAR was carried out using 25-μm thick sections. Results: Fusion images allowed direct comparison of 14C-CHC uptake in tumor and nontumor tissues. Mean 14C-CHC distribution in sensitive and resistant tumors was 882.0 ± 43.6 and 399.6 ± 157.7 nCi/g corresponding to 24.5 ± 1.21 and 11.1 ± 4.38 nmole/g, respectively (p < 0.001), with normal tissue distribution in both groups being similar. Three- dimensional QAR showed that the uptake of 14C-CHC was in the cellular zones of the tumor. This method has potential in biodistribution studios of novel radiopharmaceuticals such as 14C-CHC. Conclusion: These studies further suggest that PET imaging of 14C-CHC is feasible to distinguish between sensitive and resistant tumor deposits in vivo. |
| Keywords: | immunohistochemistry; human cell; nonhuman; cancer radiotherapy; positron emission tomography; animal cell; animals; mice; animal experiment; animal model; tumor xenograft; drug resistance; mice, inbred balb c; whole body radiation; drug distribution; tissue distribution; mice, nude; neuroblastoma; neuroblastoma cell; contrast enhancement; transplantation, heterologous; neoplasm transplantation; drug sensitivity; autoradiography; multidrug resistance; electrophoretic mobility; colchicine; drug resistance, multiple; tomography, emission-computed; biodistribution; carbon 14; carbon radioisotopes; human; priority journal; article; three-dimensional fusion imaging |
| Journal Title: | Journal of Nuclear Medicine |
| Volume: | 37 |
| Issue: | 2 |
| ISSN: | 0161-5505 |
| Publisher: | Society of Nuclear Medicine |
| Date Published: | 1996-02-01 |
| Start Page: | 312 |
| End Page: | 314 |
| Language: | English |
| PUBMED: | 8667068 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
