The effects of Flk-2/flt3 ligand as compared with c-kit ligand on short-term and long-term proliferation of CD34+ hematopoietic progenitors elicited from human fetal liver, umbilical cord blood, bone marrow, and mobilized peripheral blood Journal Article
| Authors: | Shapiro, F.; Pytowski, B.; Rafii, S.; Witte, L.; Hicklin, D. J.; Yao, T. J.; Moore, M. A. S. |
| Article Title: | The effects of Flk-2/flt3 ligand as compared with c-kit ligand on short-term and long-term proliferation of CD34+ hematopoietic progenitors elicited from human fetal liver, umbilical cord blood, bone marrow, and mobilized peripheral blood |
| Abstract: | The Flk-2/flt3 ligand (FL) was evaluated and compared with c-kit ligand (KL) for its in vitro proliferative effects on CD34+ cells from human fetal liver, umbilical cord blood, bone marrow, and mobilized peripheral blood. Using a 7-day liquid culture system, FL in combination with interleukin-3 (IL-3), interleukin-6 (IL-6), and granulocyte colony-stimulating factor (G-CSF) was comparable with KL in combination with IL-3, IL-6, and G-CSF for the expansion of hematopoietic progenitors. When FL-containing cultures were assayed after 21 or 28 days, a greater number of progenitors were generated as compared with KL-containing cultures. Using bone marrow microvascular endothelial cells as support stroma, cultures supplemented with FL generated a greater number of progenitors in both the nonadherent and adherent layers at day 35. These data suggest that FL ligand, in combination with other cytokines, can be used for short-term ex vivo expansion of hematopoietic progenitors and facilitates the preservation and possible expansion of primitive cells capable of long-term generation of progenitors. |
| Keywords: | human cell; cell proliferation; bone marrow cells; cells, cultured; cell division; stem cell factor; stem cell factor receptor; membrane proteins; protein tyrosine kinase; liver; fetal blood; organ specificity; hematopoietic stem cells; umbilical cord blood; pregnancy; blood cells; hematopoiesis; hematopoietic stem cell; antigens, cd34; receptor subtype; fetus liver; humans; human; female; priority journal; article |
| Journal Title: | Journal of Hematotherapy and Stem Cell Research |
| Volume: | 5 |
| Issue: | 6 |
| ISSN: | 1525-8165 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 1996-12-01 |
| Start Page: | 655 |
| End Page: | 662 |
| Language: | English |
| PUBMED: | 9117254 |
| PROVIDER: | scopus |
| DOI: | 10.1089/scd.1.1996.5.655 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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