Plasma lipopolysaccharide-binding protein is found associated with a particle containing apolipoprotein A-I, phospholipid, and factor H-related proteins Journal Article
| Authors: | Park, C. T.; Wright, S. D. |
| Article Title: | Plasma lipopolysaccharide-binding protein is found associated with a particle containing apolipoprotein A-I, phospholipid, and factor H-related proteins |
| Abstract: | Neutrophils exhibit a dramatic enhancement of integrin-mediated cell adhesion in response to lipopolysaccharide (LPS). This response requires CD14 on the neutrophil and plasma proteins in solution. We have purified the factor from plasma that facilitates the adhesive response of neutrophil to LPS by using a combination of affinity and ion-exchange chromatography. Previous work has shown that the activity is associated with apolipoprotein A-I (apoA-I), and here we show that this activity is associated with an apoA- I-bearing complex of protein and phospholipid. Native polyacrylamide gel electrophoresis (PAGE) analysis showed a ladder of bands in the M(r) 200,000 region, and electron microscopy revealed round, indented particles of 11.4 ± 0.12 nm in diameter. Characterization of these particles revealed a density of 1.219-1.264 g/ml and ~10 molecules of lipid phosphate per M(r) 200,000 complex. SDS-PAGE showed that each of the bands seen in native PAGE was composed of several polypeptides. These were identified as apoA-I, LPS binding protein (LBP), and factor H-related proteins (FHRPs). Physical association of apoA-I, LBP, and FHRP in these particles was further confirmed using double immunodiffusion, and association of LBP and FHRP in plasma was confirmed by co-immunoprecipitation. FHRPs are the numerically dominant protein components in these particles, and all plasma FHRP-1 appears to be associated with these particles. We suggest that FHRPs may be the defining constituent of this novel 'lipoprotein' particle. |
| Keywords: | human cell; blood proteins; protein protein interaction; amino acid sequence; molecular sequence data; neutrophil; membrane glycoproteins; carrier proteins; neutrophils; cell adhesion; blood level; phospholipids; antigens, cd14; phospholipid; lipopolysaccharides; complement factor h; apolipoprotein a1; lipoproteins, hdl; leukocyte adherence; apolipoprotein a-i; endotoxemia; acute-phase proteins; humans; human; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 271 |
| Issue: | 30 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1996-07-26 |
| Start Page: | 18054 |
| End Page: | 18060 |
| Language: | English |
| DOI: | 10.1074/jbc.271.30.18054 |
| PUBMED: | 8663389 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work