τ protects β in the leading-strand polymerase complex at the replication fork Journal Article
| Authors: | Kim, S.; Dallmann, H. G.; McHenry, C. S.; Marians, K. J. |
| Article Title: | τ protects β in the leading-strand polymerase complex at the replication fork |
| Abstract: | Replication forks formed in the absence of the τ subunit of the DNA polymerase III holoenzyme produce shorter leading and lagging strands than when τ is present. We show that one reason for this is that in the absence of τ, but in the presence of the γ-complex, leading-strand synthesis is no longer highly processive. In the absence of τ, the size of the leading strand becomes proportional to the concentration of β and inversely proportional to the concentration of the γ-complex. In addition, the β in the leading-strand complex is no longer resistant to challenge by either anti-β antibodies or poly(dA):oligo(dT). Thus, τ is required to cement a processive leading-strand complex, presumably by preventing removal of β catalyzed by the γ-complex. |
| Keywords: | dna replication; dna synthesis; transcription factors; kinetics; escherichia coli; helicase; dna, bacterial; dna conformation; dna strand; dna directed dna polymerase gamma; templates, genetic; replication origin; dna polymerase iii; dna, circular; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 271 |
| Issue: | 8 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1996-02-23 |
| Start Page: | 4315 |
| End Page: | 4318 |
| Language: | English |
| DOI: | 10.1074/jbc.271.8.4315 |
| PUBMED: | 8626779 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
