Expression and sequence analysis of the SDI1/WAF1/CIP1/p21 tumor suppressor gene in prostate cancer cell lines Journal Article

  


Authors: Papandreou, C. N.; Bogenrieder, T.; Scher, H. I.; Albino, A. P.; Nanus, D. M.
Article Title: Expression and sequence analysis of the SDI1/WAF1/CIP1/p21 tumor suppressor gene in prostate cancer cell lines
Abstract: The SDI1/WAF1/CIP1 gene encodes a M(r) 21,000 protein (p21) that can arrest cell growth by associating with and inhibiting cyclin-dependent kinase complexes necessary for cells to exit G1. It is a critical downstream effector in the p53 growth control pathway and can be transcriptionally upregulated by increasing levels of wild-type p53 protein. Somatic mutations in the p21 gene have been detected in 17% of primary prostate cancers. In the current study, we examined four prostate cancer cell lines for expression of and mutations in the p21 gene. Transcripts for p21 mRNA were present in all PC cell lines; p21 protein was detected in androgen-dependent LNCaP cells, as well as in androgen-independent DU-145 and TSU-Pr1 cells but not in androgen-independent PC-3 cells. Examination of the entire coding region of the p21 gene by SSCP analysis and direct DNA sequencing did not detect mutations in the coding domains of the p21 gene. These data indicate that mutations of the SDI1/WAF1/CIP1/p21 gene are not present in cultured PC cells and suggest that defects in the p21 gene are infrequent in prostate cancers.
Keywords: controlled study; gene sequence; human cell; cell cycle; gene expression; prostate cancer; tumor suppressor gene; cyclins; protein p21; p53; p21; prostate carcinoma; human; priority journal; article
Journal Title: International Journal of Oncology
Volume: 8
Issue: 6
ISSN: 1019-6439
Publisher: Spandidos Publications  
Date Published: 1996-06-01
Start Page: 1237
End Page: 1241
Language: English
PROVIDER: scopus
PUBMED: 21544489
DOI: 10.3892/ijo.8.6.1237
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029979124&partnerID=40&md5=0bb982f09ee9dbb3e8b3f1ea4c871ea2
Notes: Source: Scopus
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MSK Authors
  1. Howard Scher
    1130 Scher
  2. Christos N Papandreou
    20 Papandreou
  3. David M. Nanus
    66 Nanus
  4. Anthony P. Albino
    111 Albino
  5. Thomas Bogenrieder
    18 Bogenrieder
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