Establishment, characterization and drug sensitivity of four new human soft tissue sarcoma cell lines Journal Article

Authors: Li, W. W.; Cordon-Cardo, C.; Chen, Q.; Jhanwar, S. C.; Bertino, J. R.
Article Title: Establishment, characterization and drug sensitivity of four new human soft tissue sarcoma cell lines
Abstract: Four new cell lines were established from patients with soft tissue sarcomas. Drug sensitivity as well as genotypic characterization, which may be related to drug sensitivity in these cell lines, was determined. Karyotype, H-ras, c-myc and mutant p53 gene expression, Rb, G1- and S-phase cyclins, E2F and major cyclin/CDK inhibitors such as p16 and p21 and p-glycoprotein were analyzed using cytogenetic, Northern blot and immunological methods. Drug sensitivity was determined using growth inhibition tests. These cell lines differed in their morphology and growth rates, forming colonies in soft agar with a cloning efficiency of 4.3-13.4%, and 3 of the 4 cell lines grew in nude mice. Cytogenetic analysis of cell lines revealed highly aneuploid karyotypes. Deletion and/or translocation of chromosome 17 was seen in HS-16, HS-18 and HS-30 cells, and both copies of chromosome 13 were lost or re-arranged in the HS-18 cell line. Mutant p53 protein was present in all 4 cell lines, HS-18 cells showed no expression of the Rb protein and high levels of expression of E2F, cyclin A, cyclin E and CDK2. HS-16 expressed a higher level of cyclin D than the other 3 cell lines. p21(waf1) expression was seen in all cell lines, but p16(ink4) was expressed only in HS-30 and HS-42 cell lines. These cell lines were sensitive to taxol and relatively resistant to methotrexate, vinblastine and 5-fluorouracil when compared with the fibrosarcoma cell line HT-1080. these new cell lines should provide a useful model for the study of soft tissue sarcomas and for evaluating new drugs or treatments.
Keywords: controlled study; human cell; doxorubicin; fluorouracil; paclitaxel; methotrexate; animals; mice; gene expression; cytogenetics; tumor cells, cultured; morphology; vinblastine; sarcoma; chromosome aberration; mice, nude; tumor cell line; dactinomycin; soft tissue sarcoma; tumor suppressor protein p53; cyclin-dependent kinase inhibitor p21; cyclins; drug sensitivity; soft tissue neoplasms; trimetrexate; edatrexate; humans; human; priority journal; article
Journal Title: International Journal of Cancer
Volume: 68
Issue: 4
ISSN: 0020-7136
Publisher: John Wiley & Sons  
Date Published: 1996-11-15
Start Page: 514
End Page: 519
Language: English
DOI: 10.1002/(sici)1097-0215(19961115)68:4<514::aid-ijc18>;2-4
PUBMED: 8945624
PROVIDER: scopus
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
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MSK Authors
  1. Weiwei Li
    51 Li
  2. Joseph Bertino
    270 Bertino
  3. Suresh C Jhanwar
    217 Jhanwar
  4. Quan Guang Chen
    8 Chen