CD2 signaling in T cells involves tyrosine phosphorylation and activation of the Tec family kinase, EMT/ITK/TSK Journal Article

  


Authors: King, P. D.; Sadra, A.; Han, A.; Liu, X. R.; Sunder-Plassmann, R.; Reinherz, E. L.; Dupont, B.
Article Title: CD2 signaling in T cells involves tyrosine phosphorylation and activation of the Tec family kinase, EMT/ITK/TSK
Abstract: Ligation of the CD2 cell surface glycoprotein expressed on T lymphocytes and NK cells induces protein tyrosine phosphorylation and activation of the Src kinases, LCK and FYN. We show here that in Jurkat T leukemia cells and in peripheral blood T cells, CD2 stimulation also leads to tyrosine phosphorylation and activation of the Tec family kinase, EMT/ITK/TSK. Activation of EMT by CD2 was induced by mitogenic pairs of CD2 mAb, certain single CD2 mAb followed by secondary antibody cross-linking, and CD58-bearing sheep red blood cells. With the use of different Jurkat cell mutants it was demonstrated that CD2-mediated activation of EMT required expression of LCK, but did not require surface expression of the CD3 ΞΆ chain. Receptor-mediated activation of LCK does not in itself lead to activation of this Tec kinase since induction of LCK by ligation of CD4 or CD5 did not result in activation of EMT. The activation of EMT during CD2 signaling suggests an important role for this kinase in CD2 co-stimulation of T cell responses.
Keywords: signal transduction; controlled study; protein phosphorylation; human cell; cd3 antigen; t lymphocyte; t-lymphocytes; protein kinases; enzyme activation; tyrosine; phosphorylation; cd2 antigen; leukemia cell; natural killer cell; protein-tyrosine kinases; immunostimulation; cd5 antigen; protein kinase; leukemia cell line; jurkat cells; t lymphocytes; cell surface protein; cross linking; antigens, cd2; humans; human; priority journal; article; co-stimulatory molecules
Journal Title: International Immunology
Volume: 8
Issue: 11
ISSN: 0953-8178
Publisher: Oxford University Press  
Date Published: 1996-11-01
Start Page: 1707
End Page: 1714
Language: English
DOI: 10.1093/intimm/8.11.1707
PUBMED: 8943565
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029850824&doi=10.1093%2fintimm%2f8.11.1707&partnerID=40&md5=1fdcb1fdb85ea1988ce344e44118c176
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
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MSK Authors
  1. Xiao-Rong Liu
    21 Liu
  2. Bo Dupont
    264 Dupont
  3. Philip D. King
    19 King
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