Apoptosis induced by X-irradiation of rec-myc cells is postmitotic and not predicted by the time after irradiation or behavior of sister cells Journal Article
| Authors: | Vidair, C. A.; Chen, C. H.; Ling, C. C.; Dewey, W. C. |
| Article Title: | Apoptosis induced by X-irradiation of rec-myc cells is postmitotic and not predicted by the time after irradiation or behavior of sister cells |
| Abstract: | Rat embryo cells expressing the c-myc oncogene (rec-myc) were studied by time-lapse microscopy to determine whether radiation-induced apoptosis occurred before or after mitosis. Following X-irradiation with 9.5 Gy, cells were imaged every 3 min for 6 days. Episodes of apoptotic blebbing were very consistent from cell to cell, lasting 30-60 min, followed by cessation of movement and cell death. In contrast, the time of initiation of apoptotic blebbing was unpredictable. At least 96% of the apoptotic episodes were postmitotic, after one to four cell divisions and 2-97 h after a given division. Sister cells often behaved differently from one another, with apoptosis in one sister occurring many h or several divisions after apoptosis in the other. Thus, the onset of radiation-induced apoptosis in rec-myc cells is not strictly programmed but may result from the segregation of chromosome aberrations in the postirradiation generations. |
| Keywords: | nonhuman; mitosis; animal cell; animals; cell death; cell division; apoptosis; cell line; transfection; time factors; chromosome aberration; kinetics; genes, myc; rat; rats; oncogene c myc; cell communication; x irradiation; embryo cell; x-rays; humans; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 56 |
| Issue: | 18 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1996-09-15 |
| Start Page: | 4116 |
| End Page: | 4118 |
| Language: | English |
| PUBMED: | 8797576 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
