Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer Journal Article
| Authors: | Leftin, A.; Ben-Chetrit, N.; Klemm, F.; Joyce, J. A.; Koutcher, J. A. |
| Article Title: | Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer |
| Abstract: | Iron-deposition is a metabolic biomarker of macrophages in both normal and pathological situations, but the presence of iron in tumor and metastasis-associated macrophages is not known. Here we mapped and quantified hemosiderin-laden macrophage (HLM) deposits in murine models of metastatic breast cancer using iron and macrophage histology, and in vivo MRI. Iron MRI detected high-iron pixel clusters in mammary tumors, lung metastasis, and brain metastasis as well as liver and spleen tissue known to contain the HLMs. Iron histology showed these regions to contain clustered macrophages identified by their common iron status and tissue-intrinsic association with other phenotypic macrophage markers. The in vivo MRI and ex vivo histological images were further processed to determine the frequencies and sizes of the iron deposits, and measure the number of HLMs in each deposit to estimate the in vivo MRI sensitivity for these cells. Hemosiderin accumulation is a macrophage biomarker and intrinsic contrast source for cellular MRI associated with the innate function of macrophages in iron metabolism systemically, and in metastatic cancer. © 2017 Leftin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Journal Title: | PLoS ONE |
| Volume: | 12 |
| Issue: | 9 |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Date Published: | 2017-09-12 |
| Start Page: | e0184765 |
| Language: | English |
| DOI: | 10.1371/journal.pone.0184765 |
| PROVIDER: | scopus |
| PMCID: | PMC5595304 |
| PUBMED: | 28898277 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 October 2017 -- Source: Scopus |
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