Efficiency of combined blocking of aerobic and glycolytic metabolism pathways in treatment of N1-S1 hepatocellular carcinoma in a rat model Journal Article

  

Authors:	Yarmohammadi, H.; Wilkins, L. R.; Erinjeri, J. P.; Novak, R. D.; Exner, A. A.; Wu, H.; Petre, E. N.; Boas, E.; Ziv, E.; Haaga, J. R.
Article Title:	Efficiency of combined blocking of aerobic and glycolytic metabolism pathways in treatment of N1-S1 hepatocellular carcinoma in a rat model
Abstract:	Background/Aim: The aim of this study was to determine whether the addition of bumetanide (BU), a glycolytic metabolism pathway inhibitor, to arterial embolization improves tumor necrosis of N1-S1 hepatocellular carcinoma in a rat model. Materials and Methods: N1-S1 tumors were surgically implanted in the liver of 14 Sprague-Dawley rats. The rats were divided into three groups: In control group (n = 5), 1 ml of normal saline was injected intra-arterially. The tumor in the transarterial embolization group (TAE, n = 4) was embolized using 10 mg of 50-150 μ polyvinyl alcohol (PVA) particles and embolization plus BU group (TAE + BU, n = 5) were embolized with 10 mg of PVA plus 0.04 mg/kg of BU. Tumor volume was measured using two-dimensional ultrasound before intervention and twice a week afterward. Relative tumor volume after the intervention was calculated as the percentage of preinterventional tumor volume. After 4 weeks of observation, the rats were sacrificed for histopathological evaluation. Results: No statistically significant difference was detected in the preintervention tumor sizes between the three groups (P > 0.05). In the control group, the relative tumor volume increased to 142.5% larger than baseline measurements. In the TAE group, the tumor volume decreased by 18.2 ± 12.2%. The tumor volume in the TAE + BU group decrease by 90.4 ± 10.2%, which was 72.2% more than in TAE only group (P < 0.0001). Histopathological evaluation demonstrated no residual tumor in the TAE + BU group. Conclusion: Tumor necrosis significantly increased in N1-S1 tumor that received BU at the time of TAE when compared to TAE alone.
Keywords:	hepatocellular carcinoma; aerobic metabolism; bumetanide; transarterial embolization; glycolytic inhibition; n1-s1
Journal Title:	Journal of Cancer Research and Therapeutics
Volume:	13
Issue:	3
ISSN:	0973-1482
Publisher:	Medknow Publications
Date Published:	2017-07-01
Start Page:	533
End Page:	537
Language:	English
DOI:	10.4103/0973-1482.172127
PROVIDER:	scopus
PUBMED:	28862222
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028746204&doi=10.4103%2f0973-1482.172127&partnerID=40&md5=34f00b3b452d602426e49db4488592cc
Notes:	Frank Edward Boas goes by his middle name in the original publication -- Article -- Export Date: 2 October 2017 -- Source: Scopus

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