E2F site in the essential promoter region does not confer S phase-specific transcription of the ABCC10 gene in human prostate cancer cells Journal Article


Authors: Dabrowska, M.; Sirotnak, F. M.
Article Title: E2F site in the essential promoter region does not confer S phase-specific transcription of the ABCC10 gene in human prostate cancer cells
Abstract: ABCC10 (MRP7) plays a role in cellular detoxification and resistance to anticancer drugs. Since ABCC10 gene transcription in human prostate cancer CWR22Rv1 cells was found dependent on E2F binding sequence motif, ABCC10 expression in G1 and S phases of the cell cycle of CWR22Rv1 cells, was analyzed. The cells were synchronized in G1 phase by double thymidine block and in S phase by thymidine/mimosine double block. ABCC10 mRNA level was found to be similar in S phase-synchronized and asynchronous cell populations. In G1 phase it decreased by 2.4- to 3-fold. It is thus inferred, that ABCC10 expression in CWR22Rv1 cells is not S phase-specific but is primarily associated with cell proliferation.
Keywords: dna binding protein; promoter region; genetics; dna-binding proteins; cell cycle; cell cycle s phase; protein binding; genetic transcription; transcription, genetic; pathology; cell line, tumor; prostatic neoplasms; promoter regions, genetic; transcription factor e2f; prostate tumor; tumor cell line; binding site; binding sites; cell cycle g1 phase; g1 phase; s phase; multidrug resistance-associated proteins; p107; e2f; e2f transcription factors; multidrug resistance protein; humans; human; male; mrp7; abcc10; non-classical e2f target gene; rbl1; abcc10 protein, human
Journal Title: Acta Biochimica Polonica
Volume: 64
Issue: 2
ISSN: 0001-527X
Publisher: Polish Biochemical Society  
Date Published: 2017-01-01
Start Page: 371
End Page: 374
Language: English
DOI: 10.18388/abp.2017_1521
PUBMED: 28612064
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 5 September 2017 -- Source: Scopus
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  1. Francis M Sirotnak
    184 Sirotnak