Polycomb responds to low levels of transcription Journal Article

Authors: Berrozpe, G.; Bryant, G. O.; Warpinski, K.; Spagna, D.; Narayan, S.; Shah, S.; Ptashne, M.
Article Title: Polycomb responds to low levels of transcription
Abstract: How is Polycomb (Pc), a eukaryotic negative regulator of transcription, targeted to specific mammalian genes? Our genome-wide analysis of the Pc mark H3K27me3 in murine cells revealed that Pc is preferentially associated with CpG island promoters of genes that are transcribed at a low level and less so with promoters of genes that are either silent or more highly expressed. Studies of the CpG island promoter of the Kit gene demonstrate that Pc is largely absent when the gene is silent in myeloid cells, as well as when the gene is highly expressed in mast cells. Manipulations that increase transcription in the former case, and reduce it in the latter, increase Pc occupancy. The average negative effect of Pc, we infer, is about 2-fold. We suggest possible biological roles for such negative effects and propose a mechanism by which Pc might be recruited to weakly transcribed genes. © 2017 The Authors
Keywords: transcription; kit gene; polycomb; enhancers; genome-wide; crispr; cpg island promoters; shrad21
Journal Title: Cell Reports
Volume: 20
Issue: 4
ISSN: 2211-1247
Publisher: Cell Press  
Date Published: 2017-07-25
Start Page: 785
End Page: 793
Language: English
DOI: 10.1016/j.celrep.2017.06.076
PROVIDER: scopus
PUBMED: 28746865
PMCID: PMC5635825
Notes: Article -- Export Date: 2 August 2017 -- Source: Scopus
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MSK Authors
  1. Mark Ptashne
    61 Ptashne
  2. Gene Bryant
    14 Bryant
  3. Daniel Spagna
    4 Spagna
  4. Shivang S Shah
    4 Shah