CryoPause: A new method to immediately initiate experiments after cryopreservation of pluripotent stem cells Journal Article
| Authors: | Wong, K. G.; Ryan, S. D.; Ramnarine, K.; Rosen, S. A.; Mann, S. E.; Kulick, A.; De Stanchina, E.; Müller, F. J.; Kacmarczyk, T. J.; Zhang, C.; Betel, D.; Tomishima, M. J. |
| Article Title: | CryoPause: A new method to immediately initiate experiments after cryopreservation of pluripotent stem cells |
| Abstract: | Human pluripotent stem cells (PSCs) provide an unlimited cell source for cell therapies and disease modeling. Despite their enormous power, technical aspects have hampered reproducibility. Here, we describe a modification of PSC workflows that eliminates a major variable for nearly all PSC experiments: the quality and quantity of the PSC starting material. Most labs continually passage PSCs and use small quantities after expansion, but the “just-in-time” nature of these experiments means that quality control rarely happens before use. Lack of quality control could compromise PSC quality, sterility, and genetic integrity, which creates a variable that might affect results. This method, called CryoPause, banks PSCs as single-use, cryopreserved vials that can be thawed and immediately used in experiments. Each CryoPause bank provides a consistent source of PSCs that can be pre-validated before use to reduce the possibility that high levels of spontaneous differentiation, contamination, or genetic integrity will compromise an experiment. © 2017 The Authors |
| Keywords: | quality control; cryopreservation; cell therapy; directed differentiation; disease modeling; human pluripotent stem cell; cell banking |
| Journal Title: | Stem Cell Reports |
| Volume: | 9 |
| Issue: | 1 |
| ISSN: | 2213-6711 |
| Publisher: | Cell Press |
| Date Published: | 2017-07-11 |
| Start Page: | 355 |
| End Page: | 365 |
| Language: | English |
| DOI: | 10.1016/j.stemcr.2017.05.010 |
| PROVIDER: | scopus |
| PMCID: | PMC5511100 |
| PUBMED: | 28602613 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 August 2017 -- Source: Scopus |
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