CryoPause: A new method to immediately initiate experiments after cryopreservation of pluripotent stem cells Journal Article


Authors: Wong, K. G.; Ryan, S. D.; Ramnarine, K.; Rosen, S. A.; Mann, S. E.; Kulick, A.; De Stanchina, E.; Müller, F. J.; Kacmarczyk, T. J.; Zhang, C.; Betel, D.; Tomishima, M. J.
Article Title: CryoPause: A new method to immediately initiate experiments after cryopreservation of pluripotent stem cells
Abstract: Human pluripotent stem cells (PSCs) provide an unlimited cell source for cell therapies and disease modeling. Despite their enormous power, technical aspects have hampered reproducibility. Here, we describe a modification of PSC workflows that eliminates a major variable for nearly all PSC experiments: the quality and quantity of the PSC starting material. Most labs continually passage PSCs and use small quantities after expansion, but the “just-in-time” nature of these experiments means that quality control rarely happens before use. Lack of quality control could compromise PSC quality, sterility, and genetic integrity, which creates a variable that might affect results. This method, called CryoPause, banks PSCs as single-use, cryopreserved vials that can be thawed and immediately used in experiments. Each CryoPause bank provides a consistent source of PSCs that can be pre-validated before use to reduce the possibility that high levels of spontaneous differentiation, contamination, or genetic integrity will compromise an experiment. © 2017 The Authors
Keywords: quality control; cryopreservation; cell therapy; directed differentiation; disease modeling; human pluripotent stem cell; cell banking
Journal Title: Stem Cell Reports
Volume: 9
Issue: 1
ISSN: 2213-6711
Publisher: Cell Press  
Date Published: 2017-07-11
Start Page: 355
End Page: 365
Language: English
DOI: 10.1016/j.stemcr.2017.05.010
PROVIDER: scopus
PMCID: PMC5511100
PUBMED: 28602613
DOI/URL:
Notes: Article -- Export Date: 1 August 2017 -- Source: Scopus
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MSK Authors
  1. Amanda Kulick
    4 Kulick
  2. Karen G Wong
    1 Wong
  3. Sean David Ryan
    1 Ryan
  4. Siera A Rosen
    1 Rosen
  5. Shannon E Mann
    1 Mann