High Levels of Id1 Expression Define B1 Type Adult Neural Stem Cells Journal Article
| Authors: | Nam, H. S.; Benezra, R. |
| Article Title: | High Levels of Id1 Expression Define B1 Type Adult Neural Stem Cells |
| Abstract: | Defining the molecular identity of stem cells may be critical for formulating a rational strategy for the therapeutic intervention of stem cell dysfunction. We find that high expression of Id1, a dominant-negative helix-loop-helix transcriptional regulator, identifies a rare population of GFAP<sup>+</sup> astrocytes with stem cell attributes among the subventricular astrocytes in the adult brain. The rare, long-lived, and relatively quiescent Id1<sup>high</sup> astrocytes with morphology characteristic of B1 type astrocytes self-renew and generate migratory neuroblasts that differentiate into olfactory bulb interneurons. Cultured Id1<sup>high</sup> neural stem cells can self-renew asymmetrically and generate a stem and a differentiated cell expressing progressively lower levels of Id1, revealing an Id1 gradient in unperturbed cells of subventricular neurogenic lineages. Moreover, Id genes are necessary to confer self-renewal capacity, a characteristic of stem cell identity. We suggest that high expression of a single transcriptional regulator, Id1, molecularly defines the long-sought-after B1 type adult neural stem cells. © 2009 Elsevier Inc. All rights reserved. |
| Keywords: | protein expression; human cell; nonhuman; animal cell; mouse; animals; mice; animal tissue; cell survival; cells, cultured; cell structure; gene expression profiling; inhibitor of differentiation 1; green fluorescent protein; astrocyte; cell renewal; neural stem cell; subventricular zone; adult stem cells; cell differentiation; mice, inbred c57bl; cell lineage; gene expression regulation, developmental; transcription regulation; interneuron; stemcell; cell migration; inhibitor of differentiation protein 1; dna helix; neurogenesis; neuroblast; antigens, differentiation; olfactory bulb; astrocytes |
| Journal Title: | Cell Stem Cell |
| Volume: | 5 |
| Issue: | 5 |
| ISSN: | 1934-5909 |
| Publisher: | Cell Press |
| Date Published: | 2009-11-06 |
| Start Page: | 515 |
| End Page: | 526 |
| Language: | English |
| DOI: | 10.1016/j.stem.2009.08.017 |
| PUBMED: | 19896442 |
| PROVIDER: | scopus |
| PMCID: | PMC2775820 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 5" - "Export Date: 30 November 2010" - "Source: Scopus" |
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