Widespread mitotic bookmarking by histone marks and transcription factors in pluripotent stem cells Journal Article


Authors: Liu, Y.; Pelham-Webb, B.; Di Giammartino, D. C.; Li, J.; Kim, D.; Kita, K.; Saiz, N.; Garg, V.; Doane, A.; Giannakakou, P.; Hadjantonakis, A. K.; Elemento, O.; Apostolou, E.
Article Title: Widespread mitotic bookmarking by histone marks and transcription factors in pluripotent stem cells
Abstract: During mitosis, transcription is halted and many chromatin features are lost, posing a challenge for the continuity of cell identity, particularly in fast cycling stem cells, which constantly balance self-renewal with differentiation. Here we show that, in pluripotent stem cells, certain histone marks and stem cell regulators remain associated with specific genomic regions of mitotic chromatin, a phenomenon known as mitotic bookmarking. Enhancers of stem cell-related genes are bookmarked by both H3K27ac and the master regulators OCT4, SOX2, and KLF4, while promoters of housekeeping genes retain high levels of mitotic H3K27ac in a cell-type invariant manner. Temporal degradation of OCT4 during mitotic exit compromises its ability both to maintain and induce pluripotency, suggesting that its regulatory function partly depends on its bookmarking activity. Together, our data document a widespread yet specific bookmarking by histone modifications and transcription factors promoting faithful and efficient propagation of stemness after cell division. © 2017 The Author(s)
Keywords: mitosis; transcription factors; reprogramming; oct4; cell identity; stemness; escs; bookmarking; h3k27ac; histone marks
Journal Title: Cell Reports
Volume: 19
Issue: 7
ISSN: 2211-1247
Publisher: Cell Press  
Date Published: 2017-05-16
Start Page: 1283
End Page: 1293
Language: English
DOI: 10.1016/j.celrep.2017.04.067
PROVIDER: scopus
PUBMED: 28514649
PMCID: PMC5495017
DOI/URL:
Notes: Article -- Export Date: 2 June 2017 -- Source: Scopus
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MSK Authors
  1. Vidur Garg
    6 Garg