GuideScan software for improved single and paired CRISPR guide RNA design Journal Article

   


Authors: Perez, A. R.; Pritykin, Y.; Vidigal, J. A.; Chhangawala, S.; Zamparo, L.; Leslie, C. S.; Ventura, A.
Article Title: GuideScan software for improved single and paired CRISPR guide RNA design
Abstract: We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- A nd paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools. © 2017 Nature America, Inc., part of Springer Nature. All rights reserved.
Keywords: genes; rna; nucleic acids; genomic regions; genome-wide screens; trie data structures
Journal Title: Nature Biotechnology
Volume: 35
Issue: 4
ISSN: 1087-0156
Publisher: Nature Publishing Group  
Date Published: 2017-04-01
Start Page: 347
End Page: 349
Language: English
DOI: 10.1038/nbt.3804
PROVIDER: scopus
PUBMED: 28263296
PMCID: PMC5607865
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017363648&doi=10.1038%2fnbt.3804&partnerID=40&md5=ed76a76a8fcd0ca8ef512f4639955316
Notes: Article -- Export Date: 2 May 2017 -- Source: Scopus
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MSK Authors
  1. Andrea Ventura
    56 Ventura
  2. Christina Leslie
    187 Leslie
  3. Joana Alves DeCampos Vidigal
    10 DeCampos Vidigal
  4. Alexandar Reinaldo Perez
    14 Perez
  5. Sagar Chhangawala
    19 Chhangawala
  6. Yury Pritykin
    18 Pritykin
  7. Lee Zamparo
    5 Zamparo
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