Bcl-2 and Bax expression in thyroid tumours. An immunohistochemical and Western blot analysis Journal Article

  


Authors: Manetto, V.; Lorenzini, R.; Cordon-Cardo, C.; Krajewski, S.; Rosai, J.; Reed, J. C.; Eusebi, V.
Article Title: Bcl-2 and Bax expression in thyroid tumours. An immunohistochemical and Western blot analysis
Abstract: Bcl-2 and Bax proteins, which are involved in repressing and promoting programmed cell death, respectively, have been investigated immunohistochemically and by Western blot analysis in a series of thyroid tumours. Three immunostaining patterns were identified. Benign lesions and well-differentiated thyroid carcinomas displayed a profile similar to that of normal follicular epithelium, in which Bcl-2 immunostaining was predominant. Thyroid carcinomas associated with an aggressive behaviour, such as the tall-cell variant of papillary carcinoma and the poorly differentiated carcinomas, co-expressed both proteins. Finally, anaplastic carcinomas expressed only the Bax protein. Western blot analyses revealed that the anti-Bcl-2 antibody recognized two bands, of molecular weights 21 kDa and 25 kDa. This was only seen in the tall-cell papillary carcinomas and in the anaplastic carcinomas.
Keywords: immunohistochemistry; adult; clinical article; human tissue; protein expression; aged; aged, 80 and over; middle aged; proto-oncogene proteins; protein bcl 2; apoptosis; tumor differentiation; carcinoma, papillary; blotting, western; antibodies, monoclonal; immunoblotting; thyroid carcinoma; thyroid neoplasms; papillary carcinoma; molecular weight; thyroid follicle; proto-oncogene proteins c-bcl-2; thyroid tumor; antibody; anaplastic carcinoma; bax; bcl-2; protein bax; bcl-2-associated x protein; humans; human; priority journal; article
Journal Title: Virchows Archiv
Volume: 430
Issue: 2
ISSN: 0945-6317
Publisher: Springer  
Date Published: 1997-03-01
Start Page: 125
End Page: 130
Language: English
DOI: 10.1007/bf01008033
PUBMED: 9083515
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030937162&doi=10.1007%2fBF01008033&partnerID=40&md5=fc9358eb123aeb5d9effb1e476c64255
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus
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MSK Authors
  1. Juan Rosai
    181 Rosai
  2. Carlos Cordon-Cardo
    612 Cordon-Cardo
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