Augmentation of blood platelet levels by intratracheal administration of an adenovirus vector encoding human thrombopoietin cDNA Journal Article
| Authors: | Cannizzo, S. J.; Frey, B. M.; Raffi, S.; Moore, M. A. S.; Eaton, D.; Suzuki, M.; Singh, R.; Mack, C. A.; Crystal, R. G. |
| Article Title: | Augmentation of blood platelet levels by intratracheal administration of an adenovirus vector encoding human thrombopoietin cDNA |
| Abstract: | This study was designed to evaluate the hypothesis that administration of a replication-deficient, recombinant adenovirus vector to the epithelial surface of the respiratory tract can be used to deliver a recombinant protein to the systemic circulation in sufficient quantities to evoke a systemic response appropriate to the recombinant protein. We administered AdCMV.TPO–an adenovirus vector containing an expression cassette coding for the human thrombopoietin (TPO) cDNA–to the respiratory epithelium of immunocompetent Balb/c mice. Over the following week, serum human TPO levels were elevated, platelet levels increased more than sixfold, and megakaryocytosis was evident in bone marrow. This strategy may be a useful approach to the nonparenteral administration of a variety of therapeutic recombinant proteins, such as those relevant to clotting, endocrine function, and bone-marrow function. © 1997 Nature Publishing Group. |
| Keywords: | protein expression; nonhuman; mouse; animals; mice; animal tissue; bone marrow cells; thrombocyte; spleen; cell line; animal experiment; mice, inbred balb c; animalia; genetic vectors; recombinant proteins; gene therapy; hematopoietic stem cells; thrombocyte count; virus recombinant; cytomegalovirus; complementary dna; mutagenesis, insertional; platelet count; adenoviridae; dna, complementary; virus vector; megakaryocytes; gene transfer techniques; thrombopoietin; adenovirus; humans; priority journal; article; lung epithelium |
| Journal Title: | Nature Biotechnology |
| Volume: | 15 |
| Issue: | 6 |
| ISSN: | 1087-0156 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1997-06-01 |
| Start Page: | 570 |
| End Page: | 573 |
| Language: | English |
| DOI: | 10.1038/nbt0697-570 |
| PUBMED: | 9181581 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
