Minimal truncation of the c-myb gene product in rapid-onset B-cell lymphoma? Journal Article
| Authors: | Jiang, W.; Kanter, M. R.; Dunkel, I.; Ramsay, R. G.; Beemon, K. L.; Hayward, W. S. |
| Article Title: | Minimal truncation of the c-myb gene product in rapid-onset B-cell lymphoma? |
| Abstract: | Oncogenic activation of c-myb by insertional mutagenesis has been implicated in rapid-onset B-cell lymphomas induced by the nonacute avian leukosis virus EU-8. In these tumors, proviruses are integrated either upstream of the c-myb coding region or within the first intron of c-myb. Tumors with either type of integration contained identical chimeric mRNAs in which the viral 5' splice site was juxtaposed to the 3' splice site of c-myb exon 2 and myb exon 1 was eliminated. Both classes of integrations generated truncated Myb proteins that were indistinguishable by Western analysis. In contrast to most other examples of c-myb activation, the truncation consisted of only 20 N-terminal amino acids and did not disrupt either the DNA binding domain near the N terminus or the negative regulatory domain near the C terminus of Myb. The significance of the 20-amino-acid Myb truncation to tumorigenesis was tested by infection of chicken embryos with retroviral vectors expressing different myb gene products. While virus expressing either wild-type c-myb or c-myb mutated at the N-terminal casein kinase II sites was only weakly oncogenic at 10 weeks, the minimally truncated myb virus induced a high incidence of rapid-onset tumors, including B-cell lymphomas, sarcomas, and adenocarcinomas. |
| Keywords: | oncoprotein; proto-oncogene proteins; sequence deletion; nonhuman; protein analysis; animals; carboxy terminal sequence; animal model; gene product; phosphorylation; time factors; avian leukosis oncovirus; avian leukosis virus; genetic vectors; molecular cloning; cloning, molecular; b cell lymphoma; lymphoma, b-cell; amino terminal sequence; protein-serine-threonine kinases; recombination, genetic; alternative splicing; trans-activators; oncogene c myb; proto-oncogene proteins c-myb; retroviridae; chromosome mapping; dna, complementary; virus carcinogenesis; virus expression; virus vector; chickens; chicken; casein kinase ii; virus integration; priority journal; article; proviruses |
| Journal Title: | Journal of Virology |
| Volume: | 71 |
| Issue: | 9 |
| ISSN: | 0022-538X |
| Publisher: | American Society for Microbiology |
| Date Published: | 1997-09-01 |
| Start Page: | 6526 |
| End Page: | 6533 |
| Language: | English |
| PUBMED: | 9261372 |
| PROVIDER: | scopus |
| PMCID: | PMC191928 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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