Adeno-associated viral vector gene transfer into leptomeningeal xenografts Journal Article
| Authors: | Rosenfeld, M. R.; Bergman, I.; Schramm, L.; Griffin, J. A.; Kaplitt, M. G.; Meneses, P. I. |
| Article Title: | Adeno-associated viral vector gene transfer into leptomeningeal xenografts |
| Abstract: | Leptomeningeal carcinomatosis is a painful and debilitating complication of cancer. Indwelling reservoirs provide continuous assess to the subarachnoid space, making leptomeningeal cancer potentially amenable to gene therapy. Adeno-associated virus (AAV) is a defective virus not associated with any human disease. We used an AAV vector to transduce medulloblastoma (DAOY) cells in a nude rat model of leptomeningeal disease. After intraventricular injection of vector carrying the bacterial lacZ gene, β-galactosidase positive cells were found in the implanted tumor and in ependymal and subependymal cells but not in underlying normal brain parenchyma. No evidence of virally-mediated toxicity was noted in the animals. The results of this pilot study demonstrate that AAV vectors may be used to transfer and express foreign genes in established leptomeningeal tumors. |
| Keywords: | controlled study; protein expression; nonhuman; animals; animal tissue; cell line; animal experiment; animal model; tumor cells, cultured; gene transfer; genetic transduction; genetic vectors; cancer genetics; kidney; xenograft; meningeal neoplasms; medulloblastoma; recombinant proteins; rat; gene therapy; beta galactosidase; transplantation, heterologous; brain cancer; rats; cerebellar neoplasms; carcinomatous meningitis; brain tumors; virus vector; gene transfer techniques; cancer transplantation; beta-galactosidase; parenchyma; virus gene; antigens, viral, tumor; injections, intraventricular; adenovirus e1a proteins; subarachnoid space; ependyma cell; leptomeningeal carcinomatosis; adeno associated virus; adeno-associated virus; dependovirus; humans; female; article |
| Journal Title: | Journal of Neuro-Oncology |
| Volume: | 34 |
| Issue: | 2 |
| ISSN: | 0167-594X |
| Publisher: | Springer |
| Date Published: | 1997-09-01 |
| Start Page: | 139 |
| End Page: | 144 |
| Language: | English |
| DOI: | 10.1023/a:1005702228721 |
| PUBMED: | 9210060 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
