Cyclin D1 and retinoblastoma protein expression in Kaposi's sarcoma Journal Article
| Authors: | Horenstein, M. G.; Cesarman, E.; Wang, X.; Linkov, I.; Prieto, V. G.; Louie, D. C. |
| Article Title: | Cyclin D1 and retinoblastoma protein expression in Kaposi's sarcoma |
| Abstract: | Cyclins are implicated in the induction and control of the cell cycle. Cyclin D1 regulates G1-phase progression by phosphorylation of the retinoblastoma protein (pRb). The Kaposi's sarcoma associated herpesvirus/human herpesvirus 8 (KSHV) contains and transcribes an open reading frame with sequence similarities to cellular D-type cyclins. The KSHV-cyclin protein is associated with kinase activity capable of phosphorylating pRb in vitro. Here, we study for the first time the endogenous cyclin D1 and Rb protein expression in Kaposi's sarcoma (KS) tissue. Twenty-four consecutive biopsies of AIDS-related (n = 21) and classical (n = 3) KS were studied by immunohistochemistry with monoclonal antibodies against cyclin D1 and pRb. We detected cyclin D1 in 1 of 13 patch/plaque stage, in 4 of 5 nodular stage and in 3 of 6 visceral KS lesions. By Western blot analysis, this cellular cyclin D1 monoclonal antibody did not cross-react with the purified KSHV cyclin protein. The pRb was consistently detected in 24 of 24 KS lesions. In summary, early KS lesions rarely have detectable expression of endogenous cyclin D1. Advanced and disseminated KS lesions tend to have overexpression of endogenous cyclin D1. Therefore, cellular cyclin D1 expression appears to correlate with tumor progression in KS. The endogenous cyclin D1 is antigenically distinct from the KSHV-cyclin homolog. The pRb, which may serve as a substrate for KSHV-cyclin, is found in all KS lesions examined. |
| Keywords: | immunohistochemistry; controlled study; human tissue; protein expression; protein phosphorylation; neoplasm staging; cell cycle; tumor biopsy; enzyme activity; monoclonal antibody; blotting, western; antibodies, monoclonal; disease progression; herpes virus; immunoblotting; kaposi sarcoma; sarcoma, kaposi; tumor growth; cyclin d1; retinoblastoma protein; phosphotransferase; cell cycle g1 phase; open reading frame; humans; human; article |
| Journal Title: | Journal of Cutaneous Pathology |
| Volume: | 24 |
| Issue: | 10 |
| ISSN: | 0303-6987 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1997-11-01 |
| Start Page: | 585 |
| End Page: | 589 |
| Language: | English |
| PUBMED: | 9449484 |
| PROVIDER: | scopus |
| DOI: | 10.1111/j.1600-0560.1997.tb01088.x |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work