Capillary electrophoresis of methotrexate polyglutamates and its application in evaluation of γ-glutamyl hydrolase activity Journal Article

  


Authors: Waltham, M. C.; Lin, S.; Li, W. W.; Göker, E.; Gritsman, H.; Tong, W. P.; Bertino, J. R.
Article Title: Capillary electrophoresis of methotrexate polyglutamates and its application in evaluation of γ-glutamyl hydrolase activity
Abstract: A rapid and sensitive procedure for the separation of methotrexate (MTX) polyglutamates using capillary electrophoresis (CE) is described as it applies to the in vitro assay of the enzyme γ-glutamyl hydrolase (GGH, EC 3.4.22.12). Distinct separation of MTX and polyglutamylated forms (up to glu4) is achieved within 10 min using a 75 μm I.D. capillary (50 cm, +25 kV), and enables quantitation of both reactant and enzyme products. As activity can be reliably determined using less than 5x105 eukaryotic cells, this new technique can be used to measure GGH in patient tumor samples and investigate the relationship between GGH levels and clinical MTX resistance.
Keywords: controlled study; human cell; methotrexate; quantitative assay; cell line; in vitro study; tumor cells, cultured; enzyme activity; cell culture; capillary electrophoresis; electrophoresis, capillary; substrate specificity; molecular structure; eukaryotic cell; technique; enzyme assay; blast cell; gamma-glutamyl hydrolase; gamma glutamyl hydrolase; polyglutamic acid; humans; human; priority journal; article; methotrexate polyglutamates; γ-glutamyl hydrolase; 4-amino-10-methylpteroylglutamate; methotrexate polyglutamate
Journal Title: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume: 689
Issue: 2
ISSN: 1570-0232
Publisher: Elsevier Science BV  
Date Published: 1997-02-21
Start Page: 387
End Page: 392
Language: English
DOI: 10.1016/s0378-4347(96)00350-7
PUBMED: 9080326
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031581908&doi=10.1016%2fS0378-4347%2896%2900350-7&partnerID=40&md5=7a6c8637ff73fe801191ae5b17e0bb62
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus
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MSK Authors
  1. Weiwei Li
    63 Li
  2. William Ping-Yiu Tong
    158 Tong
  3. Joseph Bertino
    363 Bertino
  4. Silan Lin
    14 Lin
  5. Helena N Gritsman
    6 Gritsman
  6. Erdem Göker
    24 Goker
  7. Mark C. Waltham
    23 Waltham
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