Adenomatous polyposis coli tumor suppressor protein has signaling activity in Xenopus laevis embryos resulting in the induction of an ectopic dorsoanterior axis Journal Article

   

Authors:	Vleminckx, K.; Wong, E.; Guger, K.; Rubinfeld, B.; Polakis, P.; Gumbiner, B. M.
Article Title:	Adenomatous polyposis coli tumor suppressor protein has signaling activity in Xenopus laevis embryos resulting in the induction of an ectopic dorsoanterior axis
Abstract:	Mutation in the adenomatous polyposis coli (APC) tumor suppressor gene are linked to both familial and sporadic human colon cancer. So far, a clear biological function for the APC gene product has not been determined. We assayed the activity of APC in the early Xenopus embryo, which has been established as a good model for the analysis of the signaling activity of the APC-associated protein β-catenin. When expressed in the future ventral side of a four-cell embryo, full-length APC induced a secondary dorsoanterior axis and the induction of the homeobox gene Siamois. This is similar to the phenotype previously observed for ectopic β-catenin expression. In fact, axis induction by APC required the availability of cytosolic β-catenin. These results indicate that APC has signaling activity in the early Xenopus embryo. Signaling activity resides in the central domain of the protein, a part of the molecule that is missing in most of the truncating. APC mutations is not accompanied by detectable changes in expression levels of β-catenin, indicating that it has direct positive signaling activity in addition to its role in β-catenin turnover. From these results we propose a model in which β-catenin acts as part of the Wnt/β-catenin signaling pathway, either upstream of, or in conjunction with, β-catenin.
Keywords:	signal transduction; nonhuman; phenotype; animals; cell function; protein assembly; homeodomain proteins; cloning, molecular; embryo, nonmammalian; gene expression regulation, developmental; tumor suppressor gene; amino acid sequence; molecular sequence data; colon cancer; growth regulation; trans-activators; gene induction; beta catenin; cadherins; adenomatous polyposis coli protein; oligomerization; embryonic development; genetic linkage; dna, complementary; cytoskeletal proteins; xenopus laevis; genes, homeobox; embryonic induction; xenopus proteins; homeobox; colon polyposis; genes, apc; humans; priority journal; article
Journal Title:	Journal of Cell Biology
Volume:	136
Issue:	2
ISSN:	0021-9525
Publisher:	Rockefeller University Press
Date Published:	1997-01-27
Start Page:	411
End Page:	420
Language:	English
DOI:	10.1083/jcb.136.2.411
PUBMED:	9015311
PROVIDER:	scopus
PMCID:	PMC2134811
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031058705&doi=10.1083%2fjcb.136.2.411&partnerID=40&md5=8cb4a7a0a24caabb60d0e00a771e1b0c
Notes:	Article -- Export Date: 17 March 2017 -- Source: Scopus

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