A transcription activator with restricted tissue distribution regulates cell-specific expression of α1(XI) collagen Journal Article
| Authors: | Kinoshita, A.; Greenwel, P.; Tanaka, S.; Di Liberto, M.; Yoshioka, H.; Ramirez, F. |
| Article Title: | A transcription activator with restricted tissue distribution regulates cell-specific expression of α1(XI) collagen |
| Abstract: | Diffrent regulatory programss are likely to control expression of the α1 (XI) collagen (COL11A1) gene in cartilaginous and non-cartilaginous tissues and in coordination with different collagen genes. Here, we report the identification of a cis-acting element that is required for constitutive and tissue-specific activity of the proximal COL11A1 promoter. The element binds an apparently novel activator whose expression is restricted mostly, but not exclusively, to cells of mesenchymal origin. Tramsient transfection experiments using wild-type amd mutant constructs demonstrated the critical contribution of a 45-base pair upstream element (FP9) to promoter activity. The same functional tests and DNA binding assays narrowed down the critical portion of FP9 to a 20-base pair sequence, which consists of an imperfect palindrome with strong homology to the GATA consensus motif. Despite being able to bind GATA proteins in vitro, FP9 is actually recognized by a distinct ~100-kDa polypeptide (FP9C) probably belonging to the zinc-finger family of transcription factors. FP9C binding was mostly identified in nuclei from cells of mesenchymal origin, including those actively engaged in CO11A1 transcription. A positive correlation was also established between the level of FP9C binding and the degree of cell differentiation in vitro. Thus, FP9C represents an unusual example of tissue-specific and diffrentiation-related transcription factor with overlapping expression in hard and soft connective tissues. |
| Keywords: | adult; protein expression; human cell; nonhuman; animal cell; mouse; animals; mice; gene; gene expression; embryo; transcription factor; cell differentiation; tumor cells, cultured; animalia; dna; molecular sequence data; tissue distribution; peptide fragments; rat; newborn; collagen; base sequence; binding site; base pairing; binding sites; trans-activators; sequence homology; dna binding; zinc finger protein; mesenchyme; osteoblasts; jurkat cells; transcription factor gata 1; 3t3 cells; muscle, skeletal; tissue specificity; mice, inbred c3h; promoter regions (genetics); humans; human; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 272 |
| Issue: | 50 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1997-12-12 |
| Start Page: | 31777 |
| End Page: | 31784 |
| Language: | English |
| DOI: | 10.1074/jbc.272.50.31777 |
| PUBMED: | 9395523 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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