Increased uptake and accumulation of vitamin C in human immunodeficiency virus 1-infected hematopoietic cell lines Journal Article

  

Authors:	Rivas, C. I.; Vera, J. C.; Guaiquil, V. H.; Velásquez, F. V.; Bórquez-Ojeda, O. A.; Cárcamo, J. G.; Concha, I. I.; Golde, D. W.
Article Title:	Increased uptake and accumulation of vitamin C in human immunodeficiency virus 1-infected hematopoietic cell lines
Abstract:	Vitamin C (ascorbic acid) is required for normal host defense and functions importantly in cellular redox systems. To define the interrelationship between human immunodeficiency virus (HIV) infection and vitamin C flux at the cellular level, we analyzed vitamin C uptake and its effects on virus production and cellular proliferation in HIV-infected and uninfected human lymphoid, myeloid, and mononuclear phagocyte cell lines. Chronic or acute infection of these cell lines by HIV-1 led to increased expression of glucose transporter 1, associated with increased transport and accumulation of vitamin C. Infected cells also showed increased transport of glucose analogs. Exposure to vitamin C had a complex effect on cell proliferation and viral production. Low concentrations of vitamin C increased or decreased cell proliferation depending on the cell line and either had no effect or caused increased viral production. Exposure to high concentrations of vitamin C preferentially decreased the proliferation and survival of the HIV-infected cells and caused decreased viral production. These findings indicate that HIV infection in lymphocytic, monocytic, and myeloid cell lines leads to increased expression of glucose transporter 1 and consequent increased cellular vitamin C uptake. High concentrations of vitamin C were preferentially toxic to HIV-infected host defense cell lines in vitro.
Keywords:	controlled study; human cell; human immunodeficiency virus infection; cell proliferation; cell division; cell line; drug accumulation; drug uptake; hematopoietic cell; human immunodeficiency virus; ascorbic acid; virus replication; host resistance; lymphocytes; dehydroascorbic acid; oxidation reduction reaction; hiv infections; human immunodeficiency virus 1; hiv-1; glucose transporter type 1; vitamin intake; glucose transporter; hl-60 cells; phagocytes; hexoses; monosaccharide transport proteins; humans; human; priority journal; article
Journal Title:	Journal of Biological Chemistry
Volume:	272
Issue:	9
ISSN:	0021-9258
Publisher:	American Society for Biochemistry and Molecular Biology
Date Published:	1997-02-28
Start Page:	5814
End Page:	5820
Language:	English
DOI:	10.1074/jbc.272.9.5814
PUBMED:	9038196
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031041246&doi=10.1074%2fjbc.272.9.5814&partnerID=40&md5=4eea2277052ba1333f6412d718a1fbf9
Notes:	Article -- Export Date: 17 March 2017 -- Source: Scopus

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