Changes in cyclin-dependent kinase 2 and p27(kip1) accompany glial cell differentiation of central glia-4 cells Journal Article
| Authors: | Tikoo, R.; Casaccia-Bonnefil, P.; Chao, M. V.; Koff, A. |
| Article Title: | Changes in cyclin-dependent kinase 2 and p27(kip1) accompany glial cell differentiation of central glia-4 cells |
| Abstract: | The generation of different glial cell types in the central nervous system depends upon a wide variety of proliferative and differentiative signals. Here we report that changes in the levels of cyclin-dependent kinase 2 (CDK2) and the cell cycle inhihitor p27(kip1) accompany the differentiation of central glia-4 (CG-4) progenitor cells to an astrocytic cell phenotype in the presence of fetal calf serum. Although a decrease in CDK2 levels was observed in both oligodendrocyte and astrocyte cells derived from CG-4 cells, a striking increase in the levels of p27 was observed during the differentiation of astrocyte cells. In astrocyte cell extracts, inhibition of CDK2 activity could be overcome with exogenously added cyclin E. Furthermore, depletion of p27 from astrocyte extracts lowered the amount of cyclin E required for CDK2 activation. Taken together, these results suggest that the inhibitory action of p27 upon cyclin E-CDK2 may prevent entry of cells into the S phase and regulate the progression of CG-4 cells toward an astrocytic lineage. |
| Keywords: | signal transduction; human cell; cell proliferation; animals; cell cycle proteins; cells, cultured; cell cycle; enzyme inhibition; astrocyte; cell differentiation; hela cells; enzyme analysis; protein-serine-threonine kinases; protein p27; cyclin-dependent kinase inhibitor p27; tumor suppressor proteins; glia cell; neuroglia; rats; cyclin-dependent kinases; cyclins; cyclin dependent kinase; cyclin e; astrocytes; cyclin-dependent kinase 2; oligodendroglia; microtubule-associated proteins; cdc2-cdc28 kinases; humans; human; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 272 |
| Issue: | 1 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1997-01-03 |
| Start Page: | 442 |
| End Page: | 447 |
| Language: | English |
| DOI: | 10.1074/jbc.272.1.442 |
| PUBMED: | 8995281 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
