5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II Journal Article
| Authors: | McCracken, S.; Fong, N.; Rosonina, E.; Yankulov, K.; Brothers, G.; Siderovski, D.; Hessel, A.; Foster, S.; Shuman, S.; Bentley, D. L. |
| Article Title: | 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II |
| Abstract: | We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation. |
| Keywords: | nonhuman; protein domain; animals; mice; gene targeting; carboxy terminal sequence; transcription, genetic; phosphorylation; methyltransferases; enzyme phosphorylation; amino acid sequence; molecular sequence data; rna caps; rna, messenger; recombinant proteins; glutathione transferase; binding sites; rna processing; mammals; enzyme binding; rna polymerase ii; rna splicing; rna precursors; rna transcription; guanine derivative; globins; dna-directed rna polymerases; eukaryotic initiation factor-4e; messenger rna precursor; rna methyltransferase; capped rna; rna capping; nucleotidyltransferases; peptide initiation factors; rna guanylyltransferase; humans; human; priority journal; article; chloramphenicol o-acetyltransferase; ctd; mrna cap; rna 7-methyltransferase; rna pol ii; hiv-2 |
| Journal Title: | Genes and Development |
| Volume: | 11 |
| Issue: | 24 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 1997-12-15 |
| Start Page: | 3306 |
| End Page: | 3318 |
| Language: | English |
| PUBMED: | 9407024 |
| PROVIDER: | scopus |
| PMCID: | PMC316822 |
| DOI: | 10.1101/gad.11.24.3306 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
