5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II Journal Article


Authors: McCracken, S.; Fong, N.; Rosonina, E.; Yankulov, K.; Brothers, G.; Siderovski, D.; Hessel, A.; Foster, S.; Shuman, S.; Bentley, D. L.
Article Title: 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II
Abstract: We have investigated the role of the RNA Polymerase II (Pol II) carboxy- terminal domain (CTD) in mRNA 5'-capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.
Keywords: nonhuman; protein domain; animals; mice; gene targeting; carboxy terminal sequence; transcription, genetic; phosphorylation; methyltransferases; enzyme phosphorylation; amino acid sequence; molecular sequence data; rna caps; rna, messenger; recombinant proteins; glutathione transferase; binding sites; rna processing; mammals; enzyme binding; rna polymerase ii; rna splicing; rna precursors; rna transcription; guanine derivative; globins; dna-directed rna polymerases; eukaryotic initiation factor-4e; messenger rna precursor; rna methyltransferase; capped rna; rna capping; nucleotidyltransferases; peptide initiation factors; rna guanylyltransferase; humans; human; priority journal; article; chloramphenicol o-acetyltransferase; ctd; mrna cap; rna 7-methyltransferase; rna pol ii; hiv-2
Journal Title: Genes and Development
Volume: 11
Issue: 24
ISSN: 0890-9369
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 1997-12-15
Start Page: 3306
End Page: 3318
Language: English
PUBMED: 9407024
PROVIDER: scopus
PMCID: PMC316822
DOI: 10.1101/gad.11.24.3306
DOI/URL:
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus
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  1. Stewart H Shuman
    520 Shuman