Authors: | Roy, A. L.; Du, H.; Gregor, P. D.; Novina, C. D.; Martinez, E.; Roeder, R. G. |
Article Title: | Cloning of an inr- and E-box-binding protein, TFII-I, that interacts physically and functionally with USF1 |
Abstract: | The transcription factor TFII-I has been shown to bind independently to two distinct promoter elements, a pyrimidine-rich initiator (Inr) and a recognition site (E-box) for upstream stimulatory factor 1 (USF1), and to stimulate USF1 binding to both of these sites. Here we describe the isolation of a cDNA encoding TFII-I and demonstrate that the corresponding 120 kDa polypeptide, when expressed ectopically, is capable of binding to both Inr and E-box elements. The primary structure of TFII-I reveals novel features that include six directly repeated 90 residue motifs that each possess a potential helix-loop/span-helix homology. These unique structural features suggest that TFII-I may have the capacity for multiple protein-protein and, potentially, multiple protein-DNA interactions. Consistent with this hypothesis and with previous in vitro studies, we further demonstrate that ectopic TFII-I and USF1 can act synergistically, and in some cases independently, to activate transcription in vivo through both Inr and the E-box elements of the adenovirus major late promoter. We also describe domains of USF1 that are necessary for its independent and synergistic activation functions. |
Keywords: | controlled study; unclassified drug; human cell; promoter region; dna-binding proteins; protein domain; protein dna binding; protein protein interaction; transcription initiation; protein binding; transcription factor; hela cells; transfection; transcription factors; gene expression regulation; molecular cloning; cloning, molecular; transcription regulation; amino acid sequence; molecular sequence data; protein purification; recombinant proteins; immunoblotting; binding protein; binding sites; genes, reporter; sequence homology; repetitive sequences, nucleic acid; complementary dna; pyrimidine; adenoviridae; protein dna interaction; dna, complementary; adenovirus; promoter regions (genetics); inr; humans; human; priority journal; article; adenovirus major late promoter; e-box; transcription factor tfii-i; upstream activation factor 1; transcription factor tf11 1; upstream stimulatory factors |
Journal Title: | EMBO Journal |
Volume: | 16 |
Issue: | 23 |
ISSN: | 0261-4189 |
Publisher: | Wiley Blackwell |
Date Published: | 1997-12-01 |
Start Page: | 7091 |
End Page: | 7104 |
Language: | English |
PUBMED: | 9384587 |
PROVIDER: | scopus |
PMCID: | PMC1170311 |
DOI: | 10.1093/emboj/16.23.7091 |
DOI/URL: | |
Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |