Genomic cloning and localization of CTAG, a gene encoding an autoimmunogenic cancer-testis antigen NY-ESO-1, to human chromosome Xq28 Journal Article
| Authors: | Chen, Y. T.; Boyer, A. D.; Viars, C. S.; Tsang, S.; Old, L. J.; Arden, K. C. |
| Article Title: | Genomic cloning and localization of CTAG, a gene encoding an autoimmunogenic cancer-testis antigen NY-ESO-1, to human chromosome Xq28 |
| Abstract: | CTAG was initially cloned from an esophageal squamous cell carcinoma cDNA expression library by immunoscreening with autologous patient's serum. CTAG mRNA is expressed in a proportion of human cancers in a lineage-nonspecific fashion, whereas its expression in normal tissues is restricted to testis and ovary only. This expression pattern suggests that the CTAG product (NY-ESO-1) is an aberrantly activated tumor antigen and can potentially be an antigenic target for tumor vaccination. In the present study, we isolated human genomic clones of CTAG and established its genomic organization. By somatic cell hybrid studies and fluorescence in-situ hybridization, we localized this gene to chromosome Xq28, a region that also contains members of MAGE, a gene family that encodes several immunogenic tumor antigens with the characteristic cancer/testis expression pattern. |
| Keywords: | human tissue; gene sequence; human cell; proteins; membrane proteins; ovary; cloning, molecular; fluorescence in situ hybridization; antigens, neoplasm; x chromosome; testis cancer; esophagus carcinoma; serology; testis; complementary dna; chromosome mapping; cloning; genetic organization; hybrid cells; chromosome xq; humans; human; male; priority journal; article; squamous cell carcinoma antigen |
| Journal Title: | Cytogenetics and Cell Genetics |
| Volume: | 79 |
| Issue: | 3-4 |
| ISSN: | 0301-0171 |
| Publisher: | S. Karger AG |
| Date Published: | 1997-01-01 |
| Start Page: | 237 |
| End Page: | 240 |
| Language: | English |
| PUBMED: | 9605863 |
| PROVIDER: | scopus |
| DOI: | 10.1159/000134734 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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