Authors: | Ligueros, M.; Jeoung, D.; Tang, B.; Hochhauser, D.; Reidenberg, M. M.; Sonenberg, M. |
Article Title: | Gossypol inhibition of mitosis, cyclin D1 and Rb protein in human mammary cancer cells and cyclin-D1 transfected human fibrosarcoma cells |
Abstract: | The antiproliferative effects of gossypol on human MCF-7 mammary cancer cells and cyclin D1-transfected HT-1060 human fibrosarcoma cells were investigated by cell cycle analysis and effects on the cell cycle regulatory proteins Rb and cyclin D1. Flow cytometry of MCF-7 cells at 24 h indicated that 10 μM gossypol inhibited DNA synthesis by producing a G1/S block. Western blot analysis using anti-human Rb antibodies and anti-human cyclin D1 antibodies in MCF-7 cells and high- and low-expression cyclin D1-transfected fibrosarcoma cells indicated that, after 6 h exposure, gossypol decreased the expression levels of these proteins in a dose-dependent manner. Gossypol also decreased the ratio of phosphorylated to unphosphorylated Rb protein in human mammary cancer and fibrosarcoma cell lines. Gossypol (10 μM) treated also decreased cyclin D1-associated kinase activity on histone H1 used as a substrate in MCF-7 cells. These results suggest that gossypol might suppress growth by modulating the expression of cell cycle regulatory proteins Rb and cyclin D1 and the phosphorylation of Rb protein. |
Keywords: | controlled study; protein phosphorylation; human cell; flow cytometry; mitosis; cell cycle; breast cancer; cancer cell culture; tumor cells, cultured; enzyme activity; transfection; breast neoplasms; dna; fibrosarcoma; oncogene proteins; immunoblotting; cyclin d1; cyclins; retinoblastoma protein; phosphotransferase; antibody; phosphoprotein; dna synthesis inhibition; regulator protein; mitosis inhibition; gossypol; histone h1; dna transfection; humans; human; priority journal; article; rb protein; antiproliferation; mammary cancer; phosphorylation of rb protein |
Journal Title: | British Journal of Cancer |
Volume: | 76 |
Issue: | 1 |
ISSN: | 0007-0920 |
Publisher: | Nature Publishing Group |
Date Published: | 1997-07-01 |
Start Page: | 21 |
End Page: | 28 |
Language: | English |
PUBMED: | 9218727 |
PROVIDER: | scopus |
PMCID: | PMC2223792 |
DOI: | 10.1038/bjc.1997.330 |
DOI/URL: | |
Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |