Gossypol inhibition of mitosis, cyclin D1 and Rb protein in human mammary cancer cells and cyclin-D1 transfected human fibrosarcoma cells Journal Article

   


Authors: Ligueros, M.; Jeoung, D.; Tang, B.; Hochhauser, D.; Reidenberg, M. M.; Sonenberg, M.
Article Title: Gossypol inhibition of mitosis, cyclin D1 and Rb protein in human mammary cancer cells and cyclin-D1 transfected human fibrosarcoma cells
Abstract: The antiproliferative effects of gossypol on human MCF-7 mammary cancer cells and cyclin D1-transfected HT-1060 human fibrosarcoma cells were investigated by cell cycle analysis and effects on the cell cycle regulatory proteins Rb and cyclin D1. Flow cytometry of MCF-7 cells at 24 h indicated that 10 μM gossypol inhibited DNA synthesis by producing a G1/S block. Western blot analysis using anti-human Rb antibodies and anti-human cyclin D1 antibodies in MCF-7 cells and high- and low-expression cyclin D1-transfected fibrosarcoma cells indicated that, after 6 h exposure, gossypol decreased the expression levels of these proteins in a dose-dependent manner. Gossypol also decreased the ratio of phosphorylated to unphosphorylated Rb protein in human mammary cancer and fibrosarcoma cell lines. Gossypol (10 μM) treated also decreased cyclin D1-associated kinase activity on histone H1 used as a substrate in MCF-7 cells. These results suggest that gossypol might suppress growth by modulating the expression of cell cycle regulatory proteins Rb and cyclin D1 and the phosphorylation of Rb protein.
Keywords: controlled study; protein phosphorylation; human cell; flow cytometry; mitosis; cell cycle; breast cancer; cancer cell culture; tumor cells, cultured; enzyme activity; transfection; breast neoplasms; dna; fibrosarcoma; oncogene proteins; immunoblotting; cyclin d1; cyclins; retinoblastoma protein; phosphotransferase; antibody; phosphoprotein; dna synthesis inhibition; regulator protein; mitosis inhibition; gossypol; histone h1; dna transfection; humans; human; priority journal; article; rb protein; antiproliferation; mammary cancer; phosphorylation of rb protein
Journal Title: British Journal of Cancer
Volume: 76
Issue: 1
ISSN: 0007-0920
Publisher: Nature Publishing Group  
Date Published: 1997-07-01
Start Page: 21
End Page: 28
Language: English
PUBMED: 9218727
PROVIDER: scopus
PMCID: PMC2223792
DOI: 10.1038/bjc.1997.330
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030965376&partnerID=40&md5=a55647df560d95f456a13c99cd97bbdb
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus
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MSK Authors
  1. Martin Sonenberg
    28 Sonenberg
  2. Daniel Hochhauser
    15 Hochhauser
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