Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes Journal Article

  

Authors:	Gallardo, H. F.; Tan, C.; Ory, D.; Sadelain, M.
Article Title:	Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes
Abstract:	It is essential for the study of T-sell function and the improvement of adoptive cell therapies to efficiently generate large populations of human primary T cells that reliably express foreign genes. This goal is achieved by using recombinant retroviruses pseudotyped with either the gibbon ape leukemia virus (GaLV) envelope or the vesicular stomatitis virus G (VSV-G) glycoprotein. We show here that both retroviral particles mediate stable gene transfer in CD4+ and in CD8+ peripheral blood lymphocytes cultured under optimized conditions. However, VSV-G-pseudotyped virions may cause transduction artifacts that must be carefully excluded. The VSV-G virions require 10- to 100-fold higher concentrations of infectious particles to achieve levels of gene transfer comparable to GaLV-virions. Nonetheless, the physical stability of VSV-G-coated particles enables the concentration of vital stocks to 109 infectious particles per milliliter or more.
Keywords:	human cell; cd8-positive t-lymphocytes; animals; mice; stomatitis; lung neoplasms; tumor cells, cultured; hela cells; transfection; gene transfer; genetic transduction; genetic vectors; transduction, genetic; recombinant fusion proteins; membrane glycoproteins; cd4-positive t-lymphocytes; false positive reactions; artifacts; retrovirus; 3t3 cells; dna, complementary; peripheral lymphocyte; receptor, nerve growth factor; receptors, nerve growth factor; leukemia virus, gibbon ape; virus glycoprotein; viral envelope proteins; humans; human; priority journal; article; gene products, env; vesicular stomatitis-indiana virus
Journal Title:	Blood
Volume:	90
Issue:	3
ISSN:	0006-4971
Publisher:	American Society of Hematology
Date Published:	1997-08-01
Start Page:	952
End Page:	957
Language:	English
PUBMED:	9242523
PROVIDER:	scopus
DOI:	10.1182/blood.V90.3.952
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030845528&partnerID=40&md5=a9c8439b29cfd19448a8d45a4768effb
Notes:	Article -- Export Date: 17 March 2017 -- Source: Scopus

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